D4764
Deoxyribonucleic acid from calf thymus
Genomic, unsheared
Sinónimos:
double-stranded template DNA, Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
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Número CAS:
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
293-507-3
NACRES:
NA.52
MDL number:
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Permítanos ayudarleNombre del producto
Deoxyribonucleic acid from calf thymus, Genomic, unsheared
biological source
bovine (calf) thymus
grade
Molecular Biology
form
lyophilized powder
mol wt
>20 kb
storage temp.
2-8°C
Quality Level
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Application
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay. It was used as reference to determine the DNA content in cartilage tissue digest samples.
Biochem/physiol Actions
42% GC; Tm = 87 °C.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Features and Benefits
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
• High quality template DNA
General description
High quality double-stranded template DNA isolated from the thymus of male and female calves.
Preparation Note
Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A260 units per mL. ~20 A260 units per mg DNA.
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Roel J H Custers et al.
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Small aromatic compounds like flavonoids can intercalate with DNA molecules bringing about conformational changes leading to reduced replication and transcription. Here, we have examined one dietary flavonoid, quercetin (found in many fruit and vegetables), for possible anti-cancer effects, on HeLa
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