Please consider that the condition of the stock enzyme is different from the reaction in which it is used. You will be using a small amount of enzyme in a buffered reaction that is adjusted to the optimal pH by the reaction buffer itself.
A0810
Endoglycosidase H from Streptomyces plicatus
recombinant, expressed in E. coli, buffered aqueous solution
Synonym(s):
β-N-Acetylglucosaminidase H
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RM 3,555.00
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
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Product Name
Endoglycosidase H from Streptomyces plicatus, recombinant, expressed in E. coli, buffered aqueous solution
recombinant
expressed in E. coli
conjugate
(N-linked)
form
buffered aqueous solution
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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Related Categories
1 of 4
This Item | E9762 | E0639 | E2264 |
|---|---|---|---|
| form buffered aqueous solution | form buffered aqueous solution | form solution | form solution |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| conjugate (N-linked) | conjugate (N-linked) | conjugate (N-linked) | conjugate (N-linked) |
Biochem/physiol Actions
Endoglycosidase H is involved in cleaving the N-linked glycans present between the two N-acetylglucosamine (GlcNAc) residues in the core of the glycan chain in high-mannose sugars.[1]
General description
Endoglycosidase H or endo-β-N-acetylglucosaminidase H is an glycohydrolase. It is produced by Streptomyces plicatus and other Streptomyces species.[2]
Other Notes
One unit will release N-linked oligosaccharides from 1 μmole of denatured ribonuclease B per min at 37 °C at pH 5.5.
Physical form
Solution in 20 mM Tris HCl, pH 7.5, containing 50 mM NaCl, 1 mM EDTA
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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High-Level Expression of Endo-
Freeze H H and Kranz C
Current Protocols in Molecular Biology, 0 17(3) (2010)
High-Level Expression of Endo-
Wang F, et al.
Testing, 10(3) (2015)
T Tai et al.
The Journal of biological chemistry, 250(21), 8569-8575 (1975-11-10)
Heterogeneities of the two ovalbumin glycopeptides, (Man)5(GlcNAc)2Asn and (Man)6(GlcNAc)2Asn, were revealed by borate paper electrophoresis of oligosaccharide alcohols obtained from the glycopeptides by endo-beta-N-acetylglucosaminidase H digestion and NaB3H4 reduction. The structures of the major components of the oligosaccharides were determined
Roger S Zou et al.
Aging, 3(10), 968-984 (2011-10-13)
A distinct conformational transition from the α-helix-rich cellular prion protein (PrPC) into its β-sheet-rich pathological isoform (PrPSc) is the hallmark of prion diseases, a group of fatal transmissible encephalopathies that includes spontaneous and acquired forms. Recently, a PrPSc-like intermediate form
Young-Cheon Kim et al.
Plant physiology, 161(1), 455-464 (2012-11-13)
Many plant proteins are modified with N-linked oligosaccharides at asparagine-X-serine/threonine sites during transit through the endoplasmic reticulum and the Golgi. We have identified a number of Arabidopsis (Arabidopsis thaliana) proteins with modifications consisting of an N-linked N-acetyl-D-glucosamine monosaccharide (N-GlcNAc). Electron
Articles
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
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Instructions
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Could you provide guidance on adjusting the pH of a solution that has a pH of 7.5, even though the workable pH range mentioned in the data sheet is 5.0–6.0? Specifically, which acid would be best to use for adjusting the pH before using the solution?
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