Synthetic peptide from the semaphorin domain of human plexin-A1.
Application
Research Category Neuroscience
Research Sub Category Growth Cones & Axon Guidance
This Anti-Plexin-A1 Antibody is validated for use in WB for the detection of Plexin-A1.
Western blot: 1:1,000 on neonatal rat brain and COS-7 cells transfected with mouse Plexin-A1. The antibody reacts with the ~220 kDa protein. The suggested antibody diluent is PBS containing 5% non-fat milk and 0.04% Tween 20. Other bands may be present depending on the concentration of antibody and sample used. Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Plexin-A1. The immunogen sequence has low homology with other plexin family members.
Physical form
Affinity purified immunoglobulin. Liquid in PBS containing 50% glycerol, 1 mg/mL BSA and 0.05% sodium azide.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
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Human molecular genetics, 26(11), 2006-2017 (2017-03-24)
The olfacto-genital syndrome (Kallmann syndrome) associates congenital hypogonadism due to gonadotropin-releasing hormone (GnRH) deficiency and anosmia. This is a genetically heterogeneous developmental disease with various modes of transmission, including oligogenic inheritance. Previous reports have involved defective cell signaling by semaphorin-3A
I have a question regarding this antibody, I'm planning to run a western blot analysis on zebrafish lysates for PLXNA1, and I was wondering if this antibody would be compatible with zebrafish lysates
1 answer
Technical Support
·3 years ago
This product is tested for suitability in rats and humans. Suitability in zebrafish has not been tested and would have to be determined by the end user.
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