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Merck

MAB2258

Anti-Syntaxin-3 Antibody, clone 1-146

clone 1-146, from mouse

Synonym(s):

syntaxin 3, syntaxin 3A

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100 μG

$919.00

$919.00


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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1-146, monoclonal

species reactivity

human, rat

species reactivity (predicted by homology)

mouse

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... STX3(6809)
rat ... Stx3(81802)

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This Item
MABN2394S4819HPA002191
antibody form

purified antibody

antibody form

purified antibody

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

species reactivity

human, rat

species reactivity

mouse, human, bovine

species reactivity

rat

species reactivity

human

clone

1-146, monoclonal

clone

12E5, monoclonal

clone

polyclonal

clone

polyclonal

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

rabbit

Gene Information

human ... STX3(6809)
rat ... Stx3(81802)

Gene Information

mouse ... Stx3(20908)

Gene Information

rat ... Stx7(60466)

Gene Information

human ... STX3(6809)

isotype

IgG1κ

isotype

IgG2aκ

isotype

-

isotype

-

General description

Approx. 33 kDa
Syntaxin-3, also known as Stx3, is a member of the SNARE family which are complexes involved in membrane fusion. Syntaxin-3 is expressed in epithelial cells and is found apically in MDCK and Caco-2 cells (1).

Immunogen

GST-tagged recombinant protein corresponding to residues contained within rat Syntaxin-3.

Application

Detect Syntaxin-3 using this Anti-Syntaxin-3 Antibody, clone 1-146 validated for use in WB, IP, IH.
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology

Biochem/physiol Actions

This antibody recognizes Syntaxin-3.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl with 0.05% NaN3.

Preparation Note

Maintain refrigerated at 2-8°C for 1 year from date of receipt.

Analysis Note

Control
Rat brain tissue lysate
Evaluated by Western Blot in rat brain tissue lysate.
Western Blot Analysis: : 0.5 µg/mL dilution of this antibody detected Syntaxin-3 on 15 µg of rat brain tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Adrian J Giovannone et al.
The Journal of biological chemistry, 293(15), 5478-5491 (2018-02-25)
Syntaxins are a conserved family of SNARE proteins and contain C-terminal transmembrane anchors required for their membrane fusion activity. Here we show that Stx3 (syntaxin 3) unexpectedly also functions as a nuclear regulator of gene expression. We found that alternative
Daniëlle Rianne José Verboogen et al.
eLife, 6 (2017-05-20)
SNARE proteins play a crucial role in intracellular trafficking by catalyzing membrane fusion, but assigning SNAREs to specific intracellular transport routes is challenging with current techniques. We developed a novel Förster resonance energy transfer-fluorescence lifetime imaging microscopy (FRET-FLIM)-based technique allowing
Ritika Chatterjee et al.
Traffic (Copenhagen, Denmark), 24(7), 270-283 (2023-04-28)
Intracellular membrane fusion is mediated by membrane-bridging complexes of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). SNARE proteins are one of the key players in vesicular transport. Several reports shed light on intracellular bacteria modulating host SNARE machinery to establish
Ying Hsu et al.
Molecular therapy. Nucleic acids, 31, 164-181 (2023-01-27)
Blindness in Bardet-Biedl syndrome (BBS) is caused by dysfunction and loss of photoreceptor cells in the retina. BBS10, mutations of which account for approximately 21% of all BBS cases, encodes a chaperonin protein indispensable for the assembly of the BBSome
Hiroshi Gomi et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 65(11), 637-653 (2017-09-16)
The comparative structure and expression of salivary components and vesicular transport proteins in the canine major salivary glands were investigated. Histochemical analysis revealed that the morphology of the five major salivary glands-parotid, submandibular, polystomatic sublingual, monostomatic sublingual, and zygomatic glands-was

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