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Merck

C5670

Calcium chloride

anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

Synonym(s):

CaCl2, Calcium dichloride

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About This Item

Linear Formula:
CaCl2
CAS Number:
10043-52-4
Molecular Weight:
110.98
NACRES:
NA.71
PubChem Substance ID:
24892577
UNSPSC Code:
12352207
EC Number:
233-140-8
MDL number:
MFCD00010903
Assay:
≥96.0%
Grade:
anhydrous
Form:
powder

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Product Name

Calcium chloride, anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

InChI key

UXVMQQNJUSDDNG-UHFFFAOYSA-L

InChI

1S/Ca.2ClH/h;2*1H/q+2;;/p-2

SMILES string

[Cl-].[Cl-].[Ca++]

grade

anhydrous

vapor pressure

0.01 mmHg ( 20 °C)

product line

BioReagent

assay

≥96.0%

form

powder

technique(s)

cell culture | insect: suitable
cell culture | plant: suitable

mp

772 °C (lit.)

Quality Level

200

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This Item
449709383147793639
Calcium chloride anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

Sigma-Aldrich

C5670

Calcium chloride

Calcium chloride AnhydroBeads™, −10 mesh, ≥99.9% trace metals basis

Sigma-Aldrich

449709

Calcium chloride

Calcium chloride desiccant, ACS reagent, ≥96.0%

Sigma-Aldrich

383147

Calcium chloride

Calcium chloride anhydrous, free-flowing, Redi-Dri™, ACS reagent, ≥96%

Sigma-Aldrich

793639

Calcium chloride

form

powder

form

-

form

powder

form

powder

assay

≥96.0%

assay

≥99.9% trace metals basis

assay

≥96.0%

assay

≥96%

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

grade

anhydrous

grade

-

grade

ACS reagent

grade

ACS reagent, anhydrous

mp

772 °C (lit.)

mp

772 °C (lit.)

mp

772 °C (lit.)

mp

772 °C (lit.)

vapor pressure

0.01 mmHg ( 20 °C)

vapor pressure

0.01 mmHg ( 20 °C)

vapor pressure

0.01 mmHg ( 20 °C)

vapor pressure

0.01 mmHg ( 20 °C)

Application

This product is insect cell culture tested (0.5 mg/ml) and is appropriate for insect cell culture applications. Calcium chloride is used in the preparation and transformation of competent E. coli and in the
transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. A protocol for the concentration of virus vectors that uses CaCl2 has been published.

General description

Calcium chloride is a commonly used reagent in biochemistry. Calcium plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability.1,2 Extensive reviews of the experimental measurement of biological calcium have been published.3,4Calcium chloride is used in the preparation and transformation of competent E. coli and in the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA.5 The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied.6 A protocol for the concentration of virus vectors that uses CaCl2 has been published.7CaCl2 has been used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from Escherichia coli.8 The crystallization of porcine pancreatic elastase in the presence of CaCl2 in the presence of sodium citrate reveals binding of calcium in the metal binding site of the protein.9 The effect of CaCl2 on the total fluorescence in the polymerication of the tubulin-like FtsZ division protein of Escherichia coli has been studied by a multiwell fluorescent assay.10

Preparation Note

This product is soluble in water (222 mg/ml, 2 M), yielding a clear, colorless solution.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000515815
0000515815
0000514597
0000514597
0000496226

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J Philippe et al.
International journal of obesity (2005), 39(2), 295-302 (2014-06-04)
A significant proportion of severe familial forms of obesity remain genetically elusive. Taking advantage of our unique cohort of multigenerational obese families, we aimed to assess the contribution of rare mutations in 29 common obesity-associated genes to familial obesity, and
Maheswari Jayakannan et al.
Journal of experimental botany, 66(7), 1865-1875 (2015-01-24)
The role of endogenous salicylic acid (SA) signalling cascades in plant responses to salt and oxidative stresses is unclear. Arabidopsis SA signalling mutants, namely npr1-5 (non-expresser of pathogenesis related gene1), which lacks NPR1-dependent SA signalling, and nudt7 (nudix hydrolase7), which
Mohsen Akbari et al.
Advanced functional materials, 24(26), 4060-4067 (2014-11-21)
The fabrication of cell-laden structures with anisotropic mechanical properties while having a precise control over the distribution of different cell types within the constructs is important for many tissue engineering applications. Automated textile technologies for making fabrics allow simultaneous control
Michael A Goren et al.
Nature communications, 5, 5115-5115 (2014-10-09)
Opsin, the rhodopsin apoprotein, was recently shown to be an ATP-independent flippase (or scramblase) that equilibrates phospholipids across photoreceptor disc membranes in mammalian retina, a process required for disc homoeostasis. Here we show that scrambling is a constitutive activity of
Naoya Nishitani et al.
The international journal of neuropsychopharmacology, 17(8), 1321-1326 (2014-05-24)
Several lines of evidence indicate that ketamine has a rapid antidepressant-like effect in rodents and humans, but underlying mechanisms are unclear. In the present study, we investigated the effect of ketamine on serotonin (5-HT) release in the rat prefrontal cortex
View All Related Papers

Articles

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. What is the procedure for preparing a 2 M solution using calcium chloride and calcium chloride dihydrate?

    1 answer

    1. The molecular weight for calcium chloride dihydrate is 147.01 g/mol, so a 2 M solution would be 294 g/L. Similarly, the molecular weight for calcium chloride is 110.98 g/mole, so a 2 M solution would be 222 g/L. Therefore, a 2 M solution of calcium chloride dihydrate (such as C7902) would be 294 g/L with a molecular weight of 147.01 g/mol.

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