M9281

3-Methyladenine

≥99% (HPLC), synthetic (organic), powder

• Synonym(s): 3-MA, 6-Amino-3-methylpurine
• Empirical Formula (Hill Notation): C₆H₇N₅
• CAS Number: 5142-23-4
• Molecular Weight: 149.15
• NACRES: NA.51
• PubChem Substance ID: 24897326
• UNSPSC Code: 41106305
• EC Number: 225-908-6
• MDL number: MFCD00010531
• Beilstein/REAXYS Number: 146087

Properties

• Product Name: 3-Methyladenine, autophagy inhibitor
• InChI: 1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3
• InChI key: FSASIHFSFGAIJM-UHFFFAOYSA-N
• SMILES string: Cn1cnc(N)c2ncnc12
• biological source: synthetic (organic)
• assay: ≥99% (HPLC)
• form: powder
• mp: ~300 °C (dec.) (lit.)
• solubility: DMF: 9.80-10.20 mg/mL, clear, colorless to light yellow
• storage temp.: room temp
• Quality Level: 300

Description

Application

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III Phosphatidylinositol 3-kinases (PI-3K). For use as an autophagy inhibitor, 3-MA is typically used at a concentration of 5 mM.

3-Methyladenine has been used as an autophagy inhibitor:

• to determine the expressions of autophagy-related genes (Beclin 1, light chain 3 (LC3) and p62) using the transfected human bone marrow mesenchymal stem cells (BM-MSCs)[1]
• with cisplatin to study the inhibition of autophagy influenced cisplatin-induced apoptosis in A2780cp cells[2]
• to study the interplay between colistin-induced autophagy and apoptosis[3]

Standard for detection of nucleic acid methylation during carcinogenesis.

Biochem/physiol Actions

3-Methyladenine (3-MA) is used to inhibit and study the mechanism of autophagy (lysosomal self-degradation) and apoptosis under various conditions. 3-MA inhibits autophagy by blocking autophagosome formation via the inhibition of type III phosphatidylinositol 3-kinases (PI-3K).[4]

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Preparation Note

This product is soluble in DMF (10 mg/mL; may require gentle heating). It is also soluble in water, 95% ethanol, or 1 N NaOH at 30 mg/mL with heating, but upon cooling, the product precipitates from solution. Solutions of 3-MA may be prepared in DMSO at 100 mM.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable