The protocol for this kit is lot specific and is provided in the Certificate of Analysis. See the link below for an example:
https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/SigmaABNO/RAB0312_1128J0733.pdf
RAB0312
Rat Interleukin-6 ELISA Kit
for for cell lysate and tissue lysate
Synonym(s):
IL-6, Interleukin-6
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1 KIT
$1,080.00
$1,080.00
Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)
About This Item
NACRES:
NA.32
UNSPSC Code:
41116158
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Product Name
Rat Interleukin-6 ELISA Kit, for for cell lysate and tissue lysate
species reactivity
rat
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
capture ELISA: suitable
input
sample type tissue lysate
sample type cell lysate
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 40.96-10000 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
rat ... Il6(24498)
1 of 4
This Item | RAB0311 | RAB0247 | RAB0288 |
|---|---|---|---|
| Gene Information rat ... Il6(24498) | Gene Information rat ... Il6(24498) | Gene Information rat ... Il10(25325) | Gene Information rat ... Il2(116562) |
| species reactivity rat | species reactivity rat | species reactivity rat | species reactivity rat |
| input sample type tissue lysate | input sample type plasma | input sample type tissue lysate | input sample type cell culture supernatant(s) |
| assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
| technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable |
General description
The Rat IL-6 ELSA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-6 cell lysate and tissue lysate.
Immunogen
Recombinant Rat IL-6
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Rat Interleukin-6 ELISA Kit has been used to determine the plasma level of interleukin-6 (IL-6) via ELISA.[1]
Biochem/physiol Actions
Interleukin-6 (IL-6) acts as a neuromodulation factor. IL-6 has two signalling pathways, known as classic signalling pathway and trans-signalling pathway. It has a neuroprotective role in ischemic stroke, by activating IL-6/interleukin 6 receptor (IL-6R)/signal transducers and activators of transcription 3 (STAT3) signalling pathway.[2] IL-6 is a multifunctional cytokine, which is implicated in acute inflammation.[3] IL-6 is associated with SAPHO (synovitis, acne, pustulosis, hyperostosis, and osteitis) syndrome.[4]
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
8A - Combustible corrosive hazardous materials
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Yuan B, et al.
Molecular Medicine Reports, 16(3), 2529-2537 (2017)
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Przepiera-Bkedzak H, et al.
Mediators of Inflammation, 2015, 2529-2537 (2015)
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BACKGROUND Coronavirus disease 2019 (COVID-19) is the cause of a pandemic disease, with severe acute respiratory syndrome by binding target epithelial lung cells through angiotensin-converting enzyme 2 in humans. Thus, patients with hypertension with COVID-19 could have worse prognosis. Indeed
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Good morning i'm a PhD sudent WE have bought a kit of rat interleukin 6 but WE don't fond the prospectus in the box So please if it's possible to send me the prospectus Cordially
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