At the time the User Guide was written, 100 mM was the highest concentration tested. Subsequent testing has been conducted with concentrations as high as 300 mM. Higher concentrations of imidazole are not recommended for concentration. Using imidazole concentrations above 100 mM is not expected to damage the device; however, high salt solutions, including imidazole, may lead to sub-optimal results. Sub-optimal results can include slower flow rates, poorer recovery, and increased detection background or interference in model systems.
UFC9100
Amicon® Ultra Centrifugal Filter, 100 kDa MWCO
sample volume 15 mL, regenerated cellulose membrane, MWCO 100 kDa
Synonym(s):
100 kDa MWCO, 15 mL sample volume, Centrifugal concentrator
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About This Item
UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24
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material
polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
15 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
12.1 cm (4.807 in.)
diam.
2.97 cm
filtration area
7.6 cm2
volume
15 mL
working volume
≤15 mL
15 mL
pore size
100 kDa MWCO
greener alternative category
shipped in
ambient
General description
Amicon® Ultra 15 mL centrifugal devices with Ultracel® 100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, IgG (156 kDa) at 1 mg/ml initial concentration is retained at >90% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Application
- Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipettor sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Filtration methods and membrane filter selection in nanoparticle purification and production.
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Related Content
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I see the device is only compatible less than 100 mM imidazole. However the his-tagged protein usually washed higher than 100 mM imidazole. what will happen if it is used to concentrate sample with imidazole higher than 100 mM?
1 answer-
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Can it be fit into 50ml centifuge rotor
1 answer-
Yes, Amicon Ultra-15 units are compatible with adapters for 50 ml tubes.
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Filters with smaller (MWCO) values (3K/10K) tend to produce a higher filtrate volume in a shorter time compared to those with larger MWCOs (e.g., 100K).Why do filters with smaller MWCOs appear to elute faster, even though they have smaller pore sizes?
1 answer-
Each liquid being concentrated in centrifugal devices, each type of substance in the liquid, each amount of particles in a liquid, and each initial volume, type of size, and surface area of a device is unique. Centrifuges are spun at a specific speed based on which type of centrifuge is being used. Therefore, there are many variables involved in the reduction of volume from one initial volume to another, and thus, the end result of the time it takes to reduce volume is different. We as a vendor cannot comment on a customer's specific observation and final result explaining volume reduction and time of a sample.
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The product comes with the tube and filter as one piece. Is this correct? how can i get under 100kDa liqud?
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No, the filter component is removable to facilitate sample recovery.
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Temperature limit?
1 answer-
The Amicon Ultra devices are rated for Room Temperature environments and "normal laboratory conditions". We have not studied a set limit but do know that if stored in a warehouse at higher temperatures and humidities, the devices can be affected.
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Is it reusable? How many times I can fill it up again? Amicon Ultra 15. First time I fill it up with 15 ml, and after can I use it again for other 15 ml....and again?
1 answer-
The Amicon Ultra 15 is designed for single use but can be used for repeated spins with the same sample. However, repeated use will cause clogging of the membrane, leading to slower flow rates and longer spin times.
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Hello, Are these tubes valid for AAV concentration? And if so, are these valid for all serotypes? Also, the user guide says that the membrane's material is 'Ultracel® low binding regenerated cellulose'. What's the difference with the PES membrane?
1 answer-
These devices can be used for virus concentration but are not validated for any specific type. Both PES and regenerated cellulose membranes have low binding, but PES has a faster flow rate. For more information, please refer to the technical article on the website: https://www.sigmaaldrich.com/technical-documents/technical-article/analytical-chemistry/filtration/viral-concentration-amicon-ultrafiltration
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Hi, I have plan to use the Amicon Ultra-15 100K for Exosome extraction from cell culture supernatant, could you please let me know what would be the best speed (rpm/rcf) and time for doing that? Thanks
1 answer-
Typically, Amicon-15 devices are spun at 4,000 x g (relative centrifugal force) for exosome isolation. However, spin times may need to be optimized, based on the sample type and volume. The image below demonstrated the relationship between volume and spin times. Please see the link below to review the product User Guide. More specific spin time information is available on page 3.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/395/pr05035w-ug-ms.pdfHelpful?
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What is the minimum starting volume I can load on Ultra 100K (100,000 MWCO) devices? Can I load as little as 500 µL?
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This product is intended for use with 4-15 mL initial sample volumes. The device has not been evaluated for use with volumes smaller than those recommended. While it may be possible to concentrate samples as low as 500 uL, the recovery volume cannot be predicted. Please see the link below to review an alternative Amicon device specifically designed for 500 uL samples.
https://www.sigmaaldrich.com/product/mm/ufc5100Please see the following link to review the full list of 100 kDa MWCO Amicon Ultra products:
https://www.sigmaaldrich.com/substance/amiconultracentrifugalfilter100kdamwco1234598765Helpful?
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Does the Amicon Ultra has residues that may released into the concentrated product', like Beta-Glucan?
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These devices contain trace amounts of glycerine. If this material interferes with downstream analysis, rinse the device with buffer or Milli-Q® water prior to use. Should interference continue, rinse with 0.1 N NaOH followed by a second spin of buffer or Milli-Q® water. NOTE: Do not allow the membrane to dry out following rinse steps. If the product will not be used immediately after rinsing, leave the final buffer or water on the membrane until the usage. Please see the link below to review the full product User Guide:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/395/pr05035w-ug-ms.pdfHelpful?
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