Amicon Ultra-0.5 devices can tolerate a maximum relative centrifugal force of 15,000 × g.
UFC5100
Amicon® Ultra Centrifugal Filter, 100 kDa MWCO
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 100 kDa
Sustainability features
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Synonym(s):
Centrifugal concentrator, 100 kDa MWCO, 0.5 mL sample volume
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| Size/SKU | Availability | Price |
|---|---|---|
24 pkg | Estimated to ship onOctober 19, 2026 | $201.00 |
96 pkg | Estimated to ship onOctober 19, 2026 | $632.00 |
500 pkg | Estimated to ship onOctober 19, 2026 | $2,600.00 |
About This Item
UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing
Pore size:
100 kDa MWCO
Working volume:
≤500 μL , 0.5 mL
Sterility:
non-sterile
Packaging:
pkg of 24 pkg, pkg of 500 pkg, pkg of 8 pkg, pkg of 96 pkg
Quality Segment
material
polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg, pkg of 500 pkg, pkg of 8 pkg, pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Sustainability features
parameter
0.5 mL sample volume
technique(s)
Diafiltration: suitable, protein extraction: suitable, protein purification: suitable
L
4.99 cm (1.96 in.)
diam.
10.8 mm
filtration area
1 cm2
volume
0.5 mL
working volume
≤500 μL , 0.5 mL
pore size
100 kDa MWCO
shipped in
ambient
General description
Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, IgG (156 kDa) at 1 mg/ml initial concentration is typically retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Application
- Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel® membrane
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
Features and Benefits
- Ultracel® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 500 μL down to 15 μL
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: 10 to 30 minutes
- Reverse spin capability provides consistent recoveries
Physical form
Color Code: Clear
Other Notes
Replaces: 42414
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| description sample volume 0.5 mL, regenerated cellulose membrane, MWCO 100 kDa | description sample volume 4 mL, regenerated cellulose membrane, MWCO 100 kDa | description sample volume 2 mL, regenerated cellulose membrane, MWCO 100 kDa | description sample volume 15 mL, regenerated cellulose membrane, MWCO 100 kDa |
| parameter 0.5 mL sample volume | parameter 4 mL sample volume | parameter 2 mL sample volume | parameter 15 mL sample volume |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| pore size 100 kDa MWCO | pore size 100 kDa MWCO | pore size 100 kDa MWCO | pore size 100 kDa MWCO |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| material regenerated cellulose membrane, polypropylene tube, polypropylene cap, styrene-butadiene housing | material regenerated cellulose membrane, polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose support, styrene-butadiene housing | material polypropylene tube (for collection), styrene-butadiene housing, regenerated cellulose membrane | material regenerated cellulose support, polypropylene tube, regenerated cellulose membrane, polyethylene cap, polyethylene liner, styrene-butadiene housing |
| working volume 0.5 mL , ≤500 μL | working volume 4 mL , ≤4 mL | working volume 2 mL , ≤2 mL | working volume 15 mL , ≤15 mL |
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Storage Class
11 - Combustible Solids
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What is the maximal centrifugal g-level that it can endure?
1 answer-
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Can I sterilize the Amicon® Ultra Centrifugal Filter, 100 kDa MWCO by autoclave before I use it?
1 answer-
There are no validated sterilization methods for the Amicon Ultra devices; however, they can be "sanitized" by performing a spin cycle with 70% ethanol, followed by two rinsing spins with sterile water. It is recommended to filter the concentrated sample using a sterile 0.2 µm syringe filter (e.g., SLGVR33RS) after processing on the Amicon devices.
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Can I reuse Amicon® Ultra Centrifugal Filter, 100 kDa MWCO? ?
1 answer-
This product is intended for single use. Repeated usage may result in declining performance.
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Que signifie le conditionnement de "8 pkg"? Cela correspond-il à 8 eppendorfs/filtres ?
1 answer-
Yes, "8 pkg" means a package of 8 Amicon® Ultra Centrifugal Filters.
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Is dimethylformamide chemically compatible with Amicon Ultra-0.5mL?
1 answer-
No data is presently available for compatibility with dimethylformamide.
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