A white polystyrene plate with a non-binding surface is recommended for this kit. Black plates have not been evaluated, but may have a lower signal. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/329/297/cs1040bul.pdf
CS1040
SIRT1 Assay Kit
sufficient for 100 assays
Synonym(s):
SIRT1 Activity Assay
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
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usage
sufficient for 100 assays
Quality Level
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... SIRT1(23411)
1 of 4
This Item | CS0170 | S8446 | 07-131 |
|---|---|---|---|
| usage sufficient for 100 assays | usage kit sufficient for 100 assays (1 mL) | usage - | usage - |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| Gene Information human ... SIRT1(23411) | Gene Information human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112) | Gene Information human ... SIRT1(23411) | Gene Information human ... SIRT1(23411) |
General description
The SIRT1 Assay Kit offers all the reagents required for the measurement of purified SIRT1 activity and for screening of inhibitors/activators.
The assay procedure is based on a two-step enzymatic reaction. The first step is deacetylation by SIRT1 of a substrate that contains an acetylated lysine side chain. The second step is the cleavage of the deacetylated substrate by the Developing Solution and the release of a highly fluorescent group. The measured fluorescence is directly proportional to the deacetylation activity of the enzyme in the sample.
The assay procedure is based on a two-step enzymatic reaction. The first step is deacetylation by SIRT1 of a substrate that contains an acetylated lysine side chain. The second step is the cleavage of the deacetylated substrate by the Developing Solution and the release of a highly fluorescent group. The measured fluorescence is directly proportional to the deacetylation activity of the enzyme in the sample.
Application
Biochem/physiol Actions
Sirtuins (Sir2) are an evolutionarily conserved family of NAD+ dependent histone/protein deacetylases that tightly couple the cleavage of NAD+ and the deacetylation of protein substrates. The reaction products are nicotinamide, the deacetylated product, and a novel metabolite, 2′-O-acetyl-ADP-ribose. The proteins within this family are named after the first protein discovered from this family, Sir2 (Silent Information Regulator 2). Besides gene silencing, sirtuin proteins are important in other processes such as cell cycling regulation and fatty acid metabolism. SIRT1 is the human homolog of Sir2 and the one most studied to date. SIRT1 mediates p53 dependent process, transcription regulation, muscle differentiation, adipogenesis, and protection from axonal degeneration. SIRT1 also participates in early embryogenesis, neurogenesis, and cardiogenesis.
Features and Benefits
The kit offers all the reagents required for the fast and easy measurement of purified SIRT1 activity and for screening of inhibitors/activators. Moreover, the kit contains an inhibitor (nicotinamide) and an activator (resveratrol) as negative and positive controls, respectively.
Kit Components Also Available Separately
Product No.
Description
SDS
- SIRT1 Substrate (Fluorometric) 100 μL
- Developer Solution 1.5 mL
- S8446SIRT1 human, recombinant, expressed in E. coli, N-terminal histidine tagged, ≥90% (SDS-PAGE), buffered aqueous glycerol solution 150 μgSDS
- NAD+ Solution 1 mL
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
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Fenofibrate suppresses cellular metabolic memory of high glucose in diabetic retinopathy via a sirtuin 1-dependent signalling pathway
Zhao S, et al.
Molecular Medicine Reports, 12(4), 6112-6118 (2015)
Nicotinamide phosphoribosyltransferase (Nampt) affects the lineage fate determination of mesenchymal stem cells: a possible cause for reduced osteogenesis and increased adipogenesis in older individuals
Li Y, et al.
Journal of Bone and Mineral Research, 26(11), 2656-2664 (2011)
Nicotinamide phosphoribosyltransferase is essential for IL-1a-mediated dedifferentiation of articular chondrocytes via SIRT1 and ERK complex signaling
Hong,E et al.
The Journal of Biological Chemistry (2011)
Jun Zhang et al.
Biochimica et biophysica acta, 1861(7), 1715-1728 (2017-03-17)
Myocardial fibrosis contributes to cardiac dysfunction. Astaxanthin (AST), a member of the carotenoid family, is a well-known antioxidant, but its effect on and underlying mechanisms in myocardial fibrosis are poorly understood. In vivo, myocardial fibrosis and cardiac dysfunction were induced
Haiyan Chu et al.
American journal of respiratory cell and molecular biology, 58(1), 28-39 (2017-08-12)
Pulmonary fibrosis is the leading cause of death in systemic sclerosis (SSc). Sirtuin1 (SIRT1) is a deacetylase with known antiinflammatory and antifibrotic activity in the liver, kidney, and skin. The role of SIRT1 in SSc-related pulmonary fibrosis is unknown. In
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Instructions
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Can I use black flat bottom plates with polystyrene, nonbonding surface in this measurment?
1 answer-
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CAN I USE THE SIRT1 ACTIVITY ASSAY KIT CS1040 TO ESTIMATE SIRT1 ENZYMATIC ACTIVITY IN MICE LUNG TISSUE LYSATE?
1 answer-
The kit is intended to measure purified SIRT1 activity and to screen the efficacy of inhibitors and activators. However, it has also been cited to assess SIRT1 enzymatic activity in cell lysates, including mice tissue lysates. The following citation may be of interest for reference:
Heliyon. 2024 Sep 4;10(17):e37083.
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