GF092

TRAIL Protein, Recombinant human

Human TRAIL (TNF-Related Apoptosis Inducing Ligand), also called APO2 Ligand, is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells.

• Synonym(s): Apo2 Ligand
• eCl@ss: 32160405
• NACRES: NA.75

Properties

• biological source: human
• Quality Level: 100
• Assay: >98% (SDS-PAGE and HPLC)
• manufacturer/tradename: Chemicon^®
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 ng/μg Endotoxin (of TRAIL/Apo2L; 1EU/μg)
• input: sample type hematopoietic stem cell(s)
• NCBI accession no.: NM_003810.2
• UniProt accession no.: P50591
• shipped in: dry ice
• Gene Information: human ... TNFSF10(8743)

Description

General description

Human TRAIL (TNF-Related Apoptosis Inducing Ligand), also called APO2 Ligand, is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apoptosis, is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR transmembrane family of proteins and contain a cytoplasmic "death domain" which activates the cell′s apoptotic machinery. GF092 is a 19.6 kDa protein comprising the full-length of the TNF-like extracellular domain of TRAIL.

Product Source: E.coli

Biochem/physiol Actions

Determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1.0-3.0 ng/mL.

Physical form

Sterile filtered, then lyophilized from 0.3 mg/mL in 10mM Sodium Phosphate + 110 mM NaCl.

Storage and Stability

The lyophilized protein may be stored at room temperature for a few weeks, but is best stored at -20°C. After a quick spin, reconstitute in sterile water to a concentration of 0.5-1.0 mg/mL. This solution can be diluted into other buffered solutions and stored at 4ºC for 1 week or -20ºC for future use. Reconstituted GF092 should be stored in undiluted aliquots at -20°C for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3