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A1653

Sigma-Aldrich

Albumin from human serum

lyophilized powder, ≥96% (agarose gel electrophoresis)

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Synonym(s):
HSA
CAS Number:
EC Number:
MDL number:
eCl@ss:
42010201
NACRES:
NA.25

biological source

human

Assay

≥96% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

monomer calculated mol wt 66478 Da

technique(s)

ELISA: suitable
tissue culture: suitable
western blot: suitable

impurities

HIV I and HIVII, HCV and HBsAg, tested negative

solubility

H2O: soluble 50 mg/mL

UniProt accession no.

storage temp.

2-8°C

InChI

1S/C3F8/c4-1(5,2(6,7)8)3(9,10)11

Inchi Key

QYSGYZVSCZSLHT-UHFFFAOYSA-N

Gene Information

human ... ALB(213)

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This Item
A8763A9511A1887
assay

≥96% (agarose gel electrophoresis)

assay

≥99% (agarose gel electrophoresis)

assay

≥97% (agarose gel electrophoresis)

assay

≥96% (agarose gel electrophoresis)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

monomer calculated mol wt 66478 Da

mol wt

monomer calculated mol wt 66478 Da

mol wt

monomer calculated mol wt 66478 Da

mol wt

monomer calculated mol wt 66478 Da

technique(s)

ELISA: suitable, tissue culture: suitable, western blot: suitable

technique(s)

ELISA: suitable, tissue culture: suitable, western blot: suitable

technique(s)

ELISA: suitable, tissue culture: suitable, western blot: suitable

technique(s)

ELISA: suitable, tissue culture: suitable, western blot: suitable

impurities

HIV I and HIVII, HCV and HBsAg, tested negative

impurities

HIV I and HIVII, HCV and HBsAg, tested negative

impurities

HIV I and HIVII, HCV and HBsAg, tested negative

impurities

HIV I and HIVII, HCV and HBsAg, tested negative, ≤0.007% Fatty Acids

General description

Albumin (ALB) constitutes 25% of the proteins produced by the liver. It is a protein of a single polypeptide chain with a thiol group. The ALB gene is mapped to human 4q13.3.

Application

Albumin from human serum has been used:
  • in cyclophilin isomerase assay
  • as a component in blocking solution for immunogold quantification of amino acids and proteins in complex subcellular compartments
  • in G-pseudotyped HIV (PHIV) assay mixture to study its effect on GSK364735 antiviral potency

Albumin was used to test its effect on the in vitro bactericidal activity of cefditoren against penicillin-resistant Streptococcus pneumonia.
It has been clinically used in serious and often life-threatening conditions, such as shock and blood loss due to trauma, burns, and surgery.

Biochem/physiol Actions

Albumin serves as a protein repository and functions as a binding and transport protein. Albumin binding dissolves the biomolecules in blood and ensures its effective transport. This prevents the loss of substance through excretion. Pathological conditions like vasculitis, lymphangiectasia, glomerulonephritis, and hepatic sinusoidal hypertension might result in hypoalbuminemia.
Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones, and is vital in regulating the colloidal osmotic pressures of blood. Albumin is also seen to bind to exogenous substances, particularly drugs (e.g., ibuprofen, warfarin), and strongly influence their pharmacokinetics. Oxidative stress leading to changes in the redox state of albumin has widely varied effects on its physiological function.

Quality

Remainder mostly globulins

Other Notes

View more information on human serum albumin.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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