APOAC

Annexin V-Cy3^™ Apoptosis Detection Kit

• Synonym(s): Red fluorescent Annexin V apoptosis assay
• NACRES: NA.32

Properties

• usage: sufficient for 200 tests
• Quality Level: 200
• packaging: pkg of 1 kit
• technique(s): flow cytometry: suitable
• application(s): cell analysis; detection
• detection method: fluorometric
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Features and Benefits

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL

• Rapid labeling of cells. Cell staining takes only 10 minutes.
• No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
• 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Principle

Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).

Legal Information

Cy3 is a trademark of Cytiva

Kit Components Only

• Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg

Kit Components Also Available Separately

• C5041 6-Carboxyfluorescein diacetate 10 mg

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable