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F0427

Sigma-Aldrich

Fas Ligand human

>95% (SDS-PAGE), recombinant, expressed in CHO cells, lyophilized powder

Synonym(s):
FasL
MDL number:
NACRES:
NA.32

recombinant

expressed in CHO cells

Quality Level

assay

>95% (SDS-PAGE)

form

lyophilized powder

mol wt

monomer calculated mol wt ~18 kDa
26-28 kDa by SDS-PAGE

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... FASLG(356)

General description

FASLG (Fas ligand) acts as a ligand for Fas receptor, and is a major protein involved in programmed cell death, apoptosis. Soluble Fas (sFAS) is usually detected in plasma prior to apoptosis.[1]

Application

Fas Ligand (FASLG) human has been used for-
  • the study of FASLG stimulation in luteal cells obtained from mid-corpus luteum (CL) and
  • the study of apoptosis induction and intracellular caspase-3 assay in human cells. [2][3]

Biochem/physiol Actions

FASLG (Fas ligand) and Fas receptor constitute the basic elements in apoptosis. Interaction of FASLG with Fas receptor leads to activation of caspase-8. This caspase in turn leads to activation of effector caspases such as caspase-3, -6 and -7. This cascade results in the hydrolysis of nuclear and cytoplasmic components. Expression of FASLG is induced by nuclear factor-κB (NFκB). NFκB/FASLG pathway facilitates the suppression of p,p′-DDT (dichlorodiphenoxytrichloroethane)-induced cell toxicity by vitamin C and E.[4] In CD4+ T cells, this protein is expressed on stimulus by T-cell receptor (TCR), both during normal and pathological conditions, such as alcohol exposure.[5]
Fas ligand, a protein belonging to the tumor necrosis factor (TNF) family of cytokines, induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1).

Other Notes

Human Fas Ligand, N-terminal 6X histidine-tagged, encodes amino acids 134-281.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Analysis Note

Measured by its ability to induce apoptosis in Jurkat cells.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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