F0427

Fas Ligand human

>95% (SDS-PAGE), recombinant, expressed in CHO cells, lyophilized powder

• Synonym(s): FasL
• MDL number: MFCD01092558
• NACRES: NA.32

Properties

• recombinant: expressed in CHO cells
• Quality Level: 100
• assay: >95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: monomer calculated mol wt ~18 kDa; 26-28 kDa by SDS-PAGE
• impurities: endotoxin, tested
• UniProt accession no.: P48023
• storage temp.: −20°C
• Gene Information: human ... FASLG(356)

Description

General description

FASLG (Fas ligand) acts as a ligand for Fas receptor, and is a major protein involved in programmed cell death, apoptosis. Soluble Fas (sFAS) is usually detected in plasma prior to apoptosis.[1]

Application

Fas Ligand (FASLG) human has been used for-

• the study of FASLG stimulation in luteal cells obtained from mid-corpus luteum (CL) and
• the study of apoptosis induction and intracellular caspase-3 assay in human cells. [2][3]

Biochem/physiol Actions

FASLG (Fas ligand) and Fas receptor constitute the basic elements in apoptosis. Interaction of FASLG with Fas receptor leads to activation of caspase-8. This caspase in turn leads to activation of effector caspases such as caspase-3, -6 and -7. This cascade results in the hydrolysis of nuclear and cytoplasmic components. Expression of FASLG is induced by nuclear factor-κB (NFκB). NFκB/FASLG pathway facilitates the suppression of p,p′-DDT (dichlorodiphenoxytrichloroethane)-induced cell toxicity by vitamin C and E.[4] In CD4⁺ T cells, this protein is expressed on stimulus by T-cell receptor (TCR), both during normal and pathological conditions, such as alcohol exposure.[5]

Fas ligand, a protein belonging to the tumor necrosis factor (TNF) family of cytokines, induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1).

Other Notes

Human Fas Ligand, N-terminal 6X histidine-tagged, encodes amino acids 134-281.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Analysis Note

Measured by its ability to induce apoptosis in Jurkat cells.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315 - H319
• Precautionary Statements: P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable