F1804

Monoclonal ANTI-FLAG^® M2 antibody produced in mouse

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

• Synonym(s): flag antibody, Anti-ddddk, Anti-dykddddk
• NACRES: NA.32

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: M2, monoclonal
• form: buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
• purified by: affinity chromatography (affinity tag purification)
• species reactivity: all
• concentration: 1 mg/mL
• isotype: IgG1
• immunogen sequence: DYKDDDDK
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Method of purification - Affinity tag purification

Specificity

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Western Blotting and EIA

Browse additional application references in our FLAG^® Literature portal.

Packaging

50, 200 μg

1, 5 mg

Physical form

Contains 50% glycerol in phosphate buffered saline.

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable