- F3165
F3165
Monoclonal ANTI-FLAG® M2 antibody produced in mouse
clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin (Purified IgG1 subclass)
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
purified by
using Protein A
species reactivity
all
concentration
3.8-4.2 mg/mL
technique(s)
western blot: 10 μg/mL (Protein A)
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
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This Item | F1804 | F4042 | F3040 |
|---|---|---|---|
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| antibody form purified immunoglobulin (Purified IgG1 subclass) | antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| clone M2, monoclonal | clone M2, monoclonal | clone M5, monoclonal | clone M1, monoclonal |
| form buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide) | form buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4) | form buffered aqueous solution | form buffered aqueous solution |
| species reactivity all | species reactivity all | species reactivity all | species reactivity all |
General description
F3165 is affinity purified using Protein A resin, so it contains not only the anti-FLAG M2 antibody but also small amounts of native mouse IgG, increasing its sensitivity in most applications.
Method of purification - Protein A
Immunogen
Application
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- immunofluorescence
- ELISA
- EIA
- chromatin immunoprecipitation
- electron microscopy
- flow cytometry
- supershift assays
Browse additional application references in our FLAG® Literature portal.
Preparation Note
Storage and Stability
Note: Overtime, small amounts of purified antibodies can precipitate from solution due to intermolecular hydrophobic interactions. If a precipitate is observed in this product, briefly centrifuge the vial to pellet the precipitate. Withdraw the desired volume of antibody solution from the clear supernatant for use. This should not alter the performance of the purified antibody in Western blot or immunoprecipitation applications.
Legal Information
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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