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R4752

Sigma-Aldrich

Ribonucleic acid, transfer from bovine liver

Type XI, lyophilized powder

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Synonym(s):
Transfer RNA, tRNA
CAS Number:
MDL number:

type

Type XI

Quality Level

form

lyophilized powder

storage temp.

−20°C

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This Item
R5636R1753R8508
form

lyophilized powder

form

buffered aqueous solution

form

lyophilized powder

form

buffered aqueous solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

type

Type XI

type

-

type

Type XX

type

-

General description

Transfer ribonucleic acid (tRNA) is a non-coding RNA. It is approximately 70-100 base pair long. It has a cloverleaf shaped secondary structure. It has three or four arms and a stem structure. It has minor bases like pseudouridine, dihydrouridine and methylated bases in its structure. It has a binding site for triplet nucleotide sequence and for amino acids.

Application

Ribonucleic acid, transfer from bovine liver has been used:
  • to analyze the efficiency of thioflavin T (ThT) as a fluorophore
  • to test its ability to inhibit the growth of endothelial cells
  • as a component in translation mixture
  • as a non-specific competitor RNA for protein purification studies using poly(A) tailed RNA affinity column

Ribonucleic acid, transfer from bovine liver may be used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC).

Biochem/physiol Actions

Transfer ribonucleic acid (tRNA) plays a major role in translation, a key step in protein synthesis. It decodes mRNA into protein. There are about 40-60 different tRNAs in a eukaryotic cell and fewer in bacteria. This depends on the degeneracy of the amino acid code. Each amino acid has a specific tRNA. tRNAs are charged with specific amino acid and carries them to the site of protein synthesis. It binds to the ribosome and directs the amino acid to the growing polypeptide chain. It also functions as a regulator of various biological processes. Mutations associated with tRNA is implicated in human diseases.

Unit Definition

One unit will yield an A260 of 1.0 in 1.0 mL of water (1 cm light path).

Analysis Note

This product is evaluated for amino acceptor activity using aminoacyl-tRNA synthetase and L-arginine

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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New role for tRNA and its fragment purified from human urinary bladder carcinoma conditioned medium: inhibition of endothelial cell growth
Zhao H, et al.
Journal of Cellular Biochemistry, 76(1), 109-117 (2000)
From DNA to Protein: The Transfer of Genetic Information, 217-221 (1980)
RNA-Protein Interactions : A Practical Approach, 149-150 (1998)
Approaches for monitoring nuclear translation
mRNA Processing and Metabolism, 103-113 (2004)
Molecular Biology of the Transfer RNA Revisited, 5-7 (2014)

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