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R7876

Ribonucleic acid, transfer from wheat germ

Type V, 15-19 units/mg solid

Synonym(s):

Transfer RNA, tRNA

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500 UNITS

PLN 600.00

2500 UNITS

PLN 2,108.00

10000 UNITS

PLN 4,000.00

PLN 600.00

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About This Item

CAS Number:
9014-25-9
UNSPSC Code:
12352106
NACRES:
NA.55
MDL number:
MFCD00132200

Key Documents

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Product Name

Ribonucleic acid, transfer from wheat germ, Type V, 15-19 units/mg solid

grade

Molecular Biology

type

Type V

form

lyophilized powder

specific activity

15-19 units/mg solid

storage condition

desiccated

solubility

H2O: ~2 mg/mL, clear to hazy

storage temp.

2-8°C

Quality Level

300

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R4752R8508R5636
Ribonucleic acid, transfer from wheat germ Type V, 15-19 units/mg solid

Sigma-Aldrich

R7876

Ribonucleic acid, transfer from wheat germ

Sigma-Aldrich

Sigma-Aldrich

R4752

Ribonucleic acid, transfer from bovine liver

Ribonucleic acid, transfer from baker’s yeast (S. cerevisiae) buffered aqueous solution

Sigma-Aldrich

R8508

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

Ribonucleic acid, transfer from baker’s yeast (S. cerevisiae) buffered aqueous solution

Sigma-Aldrich

R5636

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

form

lyophilized powder

form

lyophilized powder

form

buffered aqueous solution

form

buffered aqueous solution

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

H2O: ~2 mg/mL, clear to hazy

solubility

-

solubility

-

solubility

-

storage condition

desiccated

storage condition

-

storage condition

-

storage condition

-

grade

for molecular biology

grade

-

grade

for molecular biology

grade

for molecular biology

Application

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.

General description

Wheat germ tRNA contains many different specific tRNA molecules.

Other Notes

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).

Preparation Note

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000453978
0000453978
0000439799
0000439799
0000397938

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