SRP8024

TRAIL, Soluble human

recombinant, expressed in E. coli, His tagged, >98% (SDS-PAGE)

• Synonym(s): Apo-2L, CD253, CD339, TRAIL

Properties

• biological source: human
• recombinant: expressed in E. coli
• tag: His tagged
• Assay: >98% (SDS-PAGE)
• form: liquid
• mol wt: ~26 kDa
• packaging: pkg of 20 μg
• concentration: ≥0.2 mg/mL
• technique(s): activity assay: suitable; cell culture | mammalian: suitable; transfection: suitable
• impurities: <0.01 EU/μg endotoxin, tested
• color: clear
• UniProt accession no.: P50591
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... TNFSF10(8743)

Description

General description

TNF-related apoptosis-inducing ligand (TRAIL; Apo2L; CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines, TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. The gene encoding TRAIL is localized on human chromosome 3q26.31.

Application

TRAIL, soluble human has been used in cell culture, chemical transfection and in caspase-3/7 activity assay.

Biochem/physiol Actions

Trimerized TNF-related apoptosis-inducing ligand (TRAIL) triggers apoptosis upon ligation of cell surface TRAIL-receptor 1 (R1) and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC). Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-receptors 1,-2,-3,-4 {TRAIL-R1 (DR4; Apo2; CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A; TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1; LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D)} and osteoprotegerin (OPG; OCIF; TNFRSF11B).

Physical form

Solution in 20 mM HEPES, pH 7.9, 120 mM NaCl, 0.01% Tween 20, 1% sucrose, 1 mM DTT.

Other Notes

The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable