Merck
All Photos(4)

05-421

Sigma-Aldrich

Anti-PP2A Antibody, C subunit, clone 1D6

clone 1D6, Upstate®, from mouse

Synonym(s):
Replication protein C, protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform, protein phosphatase 2, catalytic subunit, alpha isoform, serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

100

forma przeciwciała

purified immunoglobulin

antibody product type

primary antibodies

klon

1D6, monoclonal

species reactivity

rat, Xenopus, human, rabbit, Saccharomyces cerevisiae, bovine, mouse

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

izotyp

IgG2bκ

numer dostępu NCBI

numer dostępu UniProt

transport

dry ice

Gene Information

human ... PPP2CA(5515)

Opis ogólny

Type 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal downmodulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.

Specyficzność

This antibody recognizes PP2A catalytic subunit, Mr 36 kDa.

Immunogen

A 16 residue synthetic peptide (C-RGEPHVTRRTPDYFL) corresponding to amino acids 295-309 of the 36 kDa catalytic subunit of human protein phosphatase 2A (PP2A). Clone 1D6.
Epitope: a.a. 295-309

Zastosowanie

Detect PP2A using this Anti-PP2A Antibody, C subunit, clone 1D6 validated for use in IC, IP & WB.
Immunoprecipitation:
4 µg of a previous lot immunoprecipitated PP2A from 500 µg of A431 RIPA lysate. Note: This antibody has been reported not to block phosphatase activity.

Immunocytochemistry:
5-10 µg/mL of a previous lot of this antibody showed positive immunostaining for PP2A in A431 cells fixed for 10 minutes with 4% paraformaldehyde in PBS.
Research Category
Signaling

Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neurodegenerative Diseases

Jakość

Routinely evaluated by western blot on human A431 or murine 3T3/NIH RIPA cell lysates.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected PP2A in human A431 and previously in murine 3T3 RIPA cell lysates.

Opis wartości docelowych

36 kDa

Postać fizyczna

Format: Purified
Protein A chromatography
Purified mouse monoclonal IgG2bκ in buffer containing 0.1M Tris-Glycine, pH 7.4, 0.15 M, NaCl, and 0.05% sodium azide. Frozen solution.

Przechowywanie i stabilność

Stable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Komentarz do analizy

Control
HeLa whole cell lysate, mouse NIH3T3 cells, A431 cell lysate.
Included Positive Antigen Control: Catalog # 12-301, non stimulated A431 lysate. Add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Kod klasy składowania

12 - Non Combustible Liquids

WGK

WGK 1

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service