16-256

Przeciwciało przeciw fosfatydyloserynie, klon 1H6, Alexa Fluor^™ 488

clone 1H6, Upstate^®, from mouse

• Synonim(y): Klon 1H6 anty-fosfatydyloseryna, Przeciwciało 488-Alexa Fluor, Wykrywanie Alexa Fluor 488
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: ALEXA FLUOR^™ 488
• Clone: 1H6, monoclonal
• Application: FACS
• Citations: 25

Właściwości

• biological source: mouse
• Quality Segment: 100
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1H6, monoclonal
• species reactivity: vertebrates
• manufacturer/tradename: Upstate^®
• technique(s): flow cytometry: suitable
• isotype: IgG
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer)

Opis

General description

Significance:
Anti-Phosphatidylserine may be used to detect translocation of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer cell membrane leaflet; it provides an alternative to Annexin V for quantitation of apoptosis.

Phosphatidylserine, or PS, is a naturally occurring, phospholipid nutrient. PS is essential to the functioning of all the cells of the body, but is most concentrated in the brain. Its relative abundance in this organ reflects its proven involvement in an assortment of nerve cell functions, including nerve transmitter release and synaptic activity. Clinical studies have suggested that PS can support brain functions that tend to decline with age.

Immunogen

Liposomes containing 70% phosphatidylserine and 30% phosphatidylglycerol.

Application

Detect Phosphatidylserine using this Anti-Phosphatidylserine Antibody, clone 1H6, Alexa Fluor 488 validated for use in FC.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Biochem/physiol Actions

Recognizes phosphatidylserine (PS) in cell membranes.

Physical form

Protein G Purified

Purified mouse monoclonal IgG in buffer containing PBS containing 1% BSA, 0.05% Tween, 0.05% sodium azide. Liquid at 4ºC.

Preparation Note

Stable for 1 year at from date of receipt.

Analysis Note

Control
Negative Control: Catalog # 16-240, Normal Mouse IgG, Alexa Fluor^™ 488-conjugate.

Flow Cytometry: 0.2 μg of this antibody detected Phosphatidylserine in fixed Jurkat cells (see page 2).

Apoptosis Assay: Time course for induction of apoptosis in Jurkat cells by staurosporine, measured using either an Anti-Phosphatidylserine, clone 1H6, Alexa Fluor^™ 488 conjugate or an Annexin V FITC conjugate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Alexa Fluor^™ is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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