219461

Cathepsin K, His•Tag^®, Human, Recombinant, E. coli

Cathepsin K, His•Tag, Human, Recombinant, E. coli, CAS 94716-09-3, of the papain superfamily of cysteine proteinases plays a role in osteoclast-mediated bone resorption and collagen degradation.

• Numer CAS: 94716-09-3
• Numer WE: 3.4.22.38 (BRENDA, IUBMB)
• NACRES: NA.77
• UNSPSC Code: 12352202
• MDL number: MFCD00011084
• Form: liquid
• Assay: ≥95% (SDS-PAGE)
• Recombinant: expressed in E. coli

Właściwości

• recombinant: expressed in E. coli
• Quality Segment: 100
• assay: ≥95% (SDS-PAGE)
• form: liquid
• specific activity: ≥900 mU/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Opis

General description

Cathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts and recent studies also suggest its expression in fibroblasts. Irregularities in cathepsin K activity, either too high or too low, contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. This recombinant enzyme contains a C-terminal His•Tag sequence.

Note: 1 mU = 1 milliunit.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM NaOAC, pH 5.5. The activation was stopped by addition of 10 mM MMTS, which can be removed under reducing conditions.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Delaisse, J.M., et al. 2003. Microsc. Res. Tech.61, 504.
Dodds, R.A. 2003. Cell Biochem. Funct.21, 231.
Everts, V., et al. 2003. Calcif. Tissue Int.73, 380.
Hou, W.S., et al. 2003. Biol. Chem.384, 891.

One unit is defined as the amount of enzyme that will hydrolyze 0.4 µmol Z-FR-AMC per min at 37°C, pH 5.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• Co się dzieje?: nwg