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Creatine Phosphokinase, Rabbit Skeletal Muscle

Name: Creatine Phosphokinase, Rabbit Skeletal Muscle
Brand: MM

Synonim(y):

Creatine Phosphokinase, Rabbit Skeletal Muscle, rCPK

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Informacje o tej pozycji

Numer CAS:

9001-15-4

Numer WE:

2.7.3.2 (BRENDA, IUBMB)

UNSPSC Code:

12352202

NACRES:

NA.21

MDL number:

MFCD00130876

Specific activity:

≥300 U/mg, 25 °C, 750 U/mg, 37 °C

Biological source:

rabbit skeletal muscle

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Właściwości

biological source

rabbit skeletal muscle

Quality Segment

200

form

lyophilized

specific activity

≥300 U/mg, 25 °C, 750 U/mg, 37 °C

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, desiccated (hygroscopic)

solubility

0.1 M imidazole buffer, pH 6.6: soluble, 0.5% NaHCO₃, pH 9.0: soluble

foreign activity

ATPase, hexokinase, myokinase <0.001%

shipped in

ambient

storage temp.

−20°C

Opis

General description

Native creatine phosphokinase from rabbit muscle. Catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.

Preparation Note

Following reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.

Other Notes

Bessman, S.P. 1987. Anal. Biochem.161, 519.

One unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Ta pozycja	C3755	238407	238395
Sigma-Aldrich 2384 Creatine Phosphokinase, Rabbit Skeletal Muscle	Sigma-Aldrich C3755 Creatine Phosphokinase from rabbit muscle	Sigma-Aldrich 238407 Izoenzym fosfokinazy kreatynowej-MM, ludzki mięsień szkieletowy	Sigma-Aldrich 238395 Creatine Phosphokinase, Porcine Heart
description -	description Type I, salt-free, lyophilized powder, ≥150 units/mg protein	description Creatine Phosphokinase-MM Isoenzyme, Human Skeletal Muscle, is a native creatine phosphokinase-MM f that catalyzes the conversion of creatine and ATP to phosphocreatine and ADP.	description Creatine Phosphokinase, Porcine Heart, CAS 9001-15-4, is a native creatine phosphokinase that catalyzes the reversible conversion of creatine and ATP to creatine phosphate and ADP.
specific activity 750 U/mg, 37 °C, ≥300 U/mg, 25 °C	specific activity ≥150 units/mg protein	specific activity ≥300 units/mL	specific activity ≥300 U/mg
biological source rabbit skeletal muscle	biological source -	biological source -	biological source -
form lyophilized	form salt-free, lyophilized powder	form liquid	form lyophilized
storage condition OK to freeze, desiccated (hygroscopic)	storage condition -	storage condition OK to freeze	storage condition desiccated (hygroscopic), OK to freeze
solubility 0.1 M imidazole buffer, pH 6.6: soluble, 0.5% NaHCO₃, pH 9.0: soluble	solubility 0.25 M glycyl-glycine, pH 7.4: soluble 5.0 mg/mL, clear, colorless to slightly yellow	solubility -	solubility aqueous buffer: soluble, water: soluble
shipped in ambient	shipped in wet ice	shipped in wet ice	shipped in ambient

Klasa składowania

11 - Combustible Solids

Co się dzieje?

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

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Certyfikaty analizy (CoA)

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