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324717

Endoglycosidase H

Cleaves high-mannose N-glycans, suitable for Mass Spectrometry, from Streptomyces plicatus, recombinant, expressed in E. coli

Synonim(y):

Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli, Endo-β-N-acetylglucosaminidase H, Endo H

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Informacje o tej pozycji

Numer CAS:
Numer WE:
MDL number:
NACRES:
NA.54
UNSPSC Code:
12352202
Specific activity:
≥3 units/mL, ≥30 units/mg protein
Recombinant:
expressed in E. coli
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Nazwa produktu

Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli, Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

recombinant

expressed in E. coli

Quality Segment

conjugate

(N-linked)

form

liquid

specific activity

≥3 units/mL, ≥30 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

technique(s)

protein mass spectrometry: suitable

foreign activity

N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

General description

Note: 1 mU = 1 milliunit.
Recombinant, streptomyces plicatus endoglycosidase H expressed in E. coli. Catalyzes the hydrolysis of asparagine-linked oligomannose and hybrid, but not complex oligosaccharides from glycoprotein. Endo H cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

Physical form

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

Other Notes

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE.
Rao, V., et al. 1995. Structure 3, 449.
Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.
Trumbly, R.J., et al. 1985. J. Biol. Chem.260, 5683.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)
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Ta pozycja
A0810324725324727
description

Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

description

recombinant, expressed in E. coli, buffered aqueous solution

description

Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli cleaves asparagine-linked or free oligomannose and hybrid. Suitable for deglycosylation of native proteins.

description

Endoglycosidase F3, Elizabethkingia meningosepticum, Recombinant, E. coli, cleaves asparagine-linked or free biantennary and triantennary complex, and Man3GlcNAc oligosaccharides from glycoproteins.

specific activity

≥3 units/mL, ≥30 units/mg protein

specific activity

-

specific activity

≥17 units/mL, ≥16 units/mg protein

specific activity

≥30 units/mg protein, ≥5 units/mL

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

form

liquid

form

buffered aqueous solution

form

liquid

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage condition

do not freeze

storage condition

-

storage condition

do not freeze

storage condition

do not freeze

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice


Klasa składowania

12 - Non Combustible Liquids

Co się dzieje?

WGK 2



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