540221

Purine Nucleoside Phosphorylase, Human, Recombinant, E. coli

• Synonim(y): PNP
• NACRES: NA.42
• UNSPSC Code: 12352202
• eCl@ss: 32150313
• Specific activity: ≥25 units/mg protein
• Recombinant: expressed in E. coli

Właściwości

• recombinant: expressed in E. coli
• Quality Segment: 100
• form: liquid
• specific activity: ≥25 units/mg protein
• manufacturer/tradename: Calbiochem^®
• shipped in: dry ice
• storage temp.: −20°C

Opis

General description

Recombinant, human purine nucleotide phosphorylase expressed in E. coli without tags. Purine nucleoside phosphorylase catalyzes the phosphorlysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides in a reversible reaction. Human PNP is a target for therapeutic T-cell immune response intervention. Useful in the detection of inorganic phosphate from biochemical reactions.

Physical form

In 50 mM Tris-HCl, pH 7.6.

Analysis Note

Single band by SDS-PAGE

Other Notes

Filgueira de Azevedo, W.Jr., et al. 2003. Biochem. Biophys. Res. Commun.309, 917.
Silva, R.G., et al. 2003. Protein Expr. Purif.27, 158.

One unit is defined as the amount of enzyme that will convert 1 µmol of MESG and 1 µmol of P_i into 1 µmol of 2-amino-6-mercapto-7-methylpurine and 1 µmol of ribose phosphate in 1 min at 25°C, pH 7.6.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• WGK: WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable