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565758

β-Secretase Substrate IV, Fluorogenic

≥95% (HPLC), lyophilized solid, β-secretase substrate, Calbiochem®

Synonim(y):

β-Secretase Substrate IV, Fluorogenic, FS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic

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Rozmiar/SKUDostępnośćCena netto
1 mg

Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o.

1020,00 zł

Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C91H129N25O25S
Numer CAS:
Masa cząsteczkowa:
2005.22
MDL number:
NACRES:
NA.77
UNSPSC Code:
12352202
Assay:
≥95% (HPLC)
Form:
lyophilized solid
Storage condition:
OK to freeze, protect from light

1020,00 zł


Dostępne do wysyłki DZISIAJSzczegóły


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Nazwa produktu

β-Secretase Substrate IV, Fluorogenic, A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP).

Quality Segment

assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

color

red

solubility

DMSO: 5 mg/mL

fluorescence

λex ~350 nm, λem ~490 nm

shipped in

ambient

storage temp.

−20°C

General description

A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate (excitation max.: ~350 nm; emission max.: ~490), based on the GVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein. Hydrolysis of the substrate at Leu-Asp bond by β-secretase results in fluorescence enhancement. Reported Kcat/km values (min-1/µM) for pro-mempasin 2 and its proteolytically activated species Leu28p-mempasin 2 are 0.044 and 0.040, respectively (at pH 4.5, 37°C).

Biochem/physiol Actions

Product does not compete with ATP.
Reversible: no
Cell permeable: no
Primary Target
β-secretase substrate

Packaging

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Preparation Note

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.

Other Notes

Ermolieff, J., et al. 2000. Biochemistry 39, 12450.
H-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)
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Ta pozycja
565767565764565780
description

A highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP).

description

An internally quenched fluorogenic peptide substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP).

description

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase.

description

The β-Secretase Inhibitor III controls the biological activity of β-Secretase. This small molecule/inhibitor is primarily used for Neuroscience applications.

form

lyophilized solid

form

solid

form

lyophilized

form

lyophilized

assay

≥95% (HPLC)

assay

≥95% (HPLC)

assay

≥95% (HPLC)

assay

≥95% (HPLC)

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage condition

protect from light, OK to freeze

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

storage condition

desiccated (hygroscopic), protect from light, OK to freeze

storage condition

desiccated (hygroscopic), OK to freeze


Klasa składowania

11 - Combustible Solids

WGK

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable



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