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Merck

648317

Tris, Hydrochloride, Molecular Biology Grade

Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

Synonim(y):

Trizma® hydrochloride, tris(Hydroxymethyl)aminomethane, HCl, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride

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Informacje o tej pozycji

Wzór liniowy:
NH2C(CH2OH)3 · HCl
Numer CAS:
Masa cząsteczkowa:
157.60
NACRES:
NA.25
UNSPSC Code:
12161705
Beilstein/REAXYS Number:
3675235
MDL number:

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SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

grade

Molecular Biology

description

RTECS - TY2900000

assay

≥99% (titration)

form

crystalline solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated (hygroscopic)

color

white

useful pH range

7.0-9.0

pKa (25 °C)

8.1

solubility

water: soluble

cation traces

heavy metals (as Pb): ≤0.0002%

absorption

≤0.05 at 260 nm, ≤0.05 at 280 nm

foreign activity

DNases, proteases, RNases, none detected

shipped in

ambient

storage temp.

15-25°C

Quality Level

Powiązane kategorie

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Ta pozycja
6483139310-OPPHR9293
assay

≥99% (titration)

assay

≥99% (titration)

assay

-

assay

-

grade

Molecular Biology

grade

-

grade

-

grade

certified reference material, TraceCERT®

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

300

form

crystalline solid

form

solid

form

crystalline powder, needles

form

-

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

-

storage temp.

2-8°C

storage condition

OK to freeze, desiccated (hygroscopic)

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

-

General description

Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
Widely used biological buffer (absorbance: ≤0.05 at 260 nm and 280 nm). Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

Other Notes

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)
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Klasa składowania

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Questions

1–3 of 3 Questions  
  1. How can i prepare the buffer HCL solution?

    1 answer
  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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