An amount of this product would need to be added to water in order to create the concentration of the desired buffer (e.g. 157.60 grams for one liter of a 1 M solution). The pH can then be adjusted as necessary.
648317
Tris, Hydrochloride, Molecular Biology Grade
Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
Synonim(y):
Trizma® hydrochloride, tris(Hydroxymethyl)aminomethane, HCl, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
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Informacje o tej pozycji
Wzór liniowy:
NH2C(CH2OH)3 · HCl
Numer CAS:
Masa cząsteczkowa:
157.60
NACRES:
NA.25
UNSPSC Code:
12161705
Beilstein/REAXYS Number:
3675235
MDL number:
grade
Molecular Biology
Quality Level
description
RTECS - TY2900000
assay
≥99% (titration)
form
crystalline solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic)
color
white
useful pH range
7.0-9.0
pKa (25 °C)
8.1
solubility
water: soluble
cation traces
heavy metals (as Pb): ≤0.0002%
absorption
≤0.05 at 260 nm, ≤0.05 at 280 nm
foreign activity
DNases, proteases, RNases, none detected
shipped in
ambient
storage temp.
15-25°C
SMILES string
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
General description
Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
Widely used biological buffer (absorbance: ≤0.05 at 260 nm and 280 nm). Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
Other Notes
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Ta pozycja | |||
|---|---|---|---|
| grade Molecular Biology | grade - | grade - | grade - |
| assay ≥99% (titration) | assay ≥99% (titration) | assay >99% (titration) | assay - |
| Quality Level 100 | Quality Level 100 | Quality Level - | Quality Level 100 |
| form crystalline solid | form solid | form solid | form crystalline powder, needles |
| storage condition OK to freeze, desiccated (hygroscopic) | storage condition OK to freeze | storage condition - | storage condition OK to freeze |
| solubility water: soluble | solubility water: 500 mg/mL | solubility - | solubility - |
Klasa składowania
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certyfikaty analizy (CoA)
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Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| 648317-100GM | 07790788052089 |
| 648317-1KG | 07790788052096 |
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How can i prepare the buffer HCL solution?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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