The minimum activity of this product is 250 U/µL. Some lots may have significantly higher activity than the specified minimum. A fixed dilution is not recommended; a 1:5000 dilution that has been used successfully on previous lots may require optimization for future lots. A review of the activity reported on the lot-specific COA is strongly recommended.
71205-M
Benzonase® Nuclease HC
Purity > 90%, Serratia marcescens, buffered aqueous glycerol solution
Synonim(y):
Endonuclease from Serratia marcescens
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
25 kU | Sprawdź dostępność w koszyku | 1250,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352202
NACRES:
NA.54
Numer WE:
MDL number:
Assay:
>90% (SDS-PAGE)
Biological source:
Serratia marcescens
Recombinant:
expressed in E. coli
Concentration:
≥250 units/μL
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócNazwa produktu
Benzonase® Nuclease HC, Purity > 90%,
biological source
Serratia marcescens
Quality Segment
recombinant
expressed in E. coli
product line
Benzonase®
assay
>90% (SDS-PAGE)
form
buffered aqueous glycerol solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
concentration
≥250 units/μL
application(s)
research use
storage temp.
−20°C
General description
Benzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens. It degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity. It is effective over a wide range of conditions and possesses an exceptionally high specific activity. The enzyme completely digests nucleic acids to 5′-monophosphate terminated oligonucleotides 2 to 5 bases in length (below the hybridization limit), which is ideal for removal of nucleic acids from recombinant proteins, enabling compliance with FDA guidelines for nucleic acid contamination. The ability of Benzonase to rapidly hydrolyze nucleic acids makes the enzyme an excellent choice for viscosity reduction to reduce processing time and increase yields of protein. For example, the enzyme is compatible with BugBuster® and PopCulture® Protein Extraction Reagents and can therefore be added along with these reagents to eliminate viscosity and remove nucleic acids from E. coli extracts.
The enzyme consists of two subunits of 30 kDa each. It is functional between pH 6 and 10 and from 0-42 °C and requires 1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF (1 mM), EDTA (1 mM) and urea (relative activity depends on specific conditions). Activity is inhibited by > 150 mM monovalent cations, > 100 mM phosphate, > 100 mM ammonium sulfate, or > 100 mM guanidine HCl.
Benzonase Nuclease is available in ultrapure (> 99% by SDS-PAGE) and pure (> 90%) grades at a standard concentration of 25-29 U/μl and at a high concentration (HC) of 250 U/μl. Both preparations are free of detectable protease and have specific activity > 1 × 106 U/mg protein. The > 99% purity grade is tested for endotoxins and contains < 0.25 EU/1000 units. The product is supplied as a 0.2 μm filtered solution in 50% glycerol. Store at -20°C.
Total endotoxin : < 0.25 EU/1,000 units. Purity : > 90% by SDS-PAGE.
The enzyme consists of two subunits of 30 kDa each. It is functional between pH 6 and 10 and from 0-42 °C and requires 1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF (1 mM), EDTA (1 mM) and urea (relative activity depends on specific conditions). Activity is inhibited by > 150 mM monovalent cations, > 100 mM phosphate, > 100 mM ammonium sulfate, or > 100 mM guanidine HCl.
Benzonase Nuclease is available in ultrapure (> 99% by SDS-PAGE) and pure (> 90%) grades at a standard concentration of 25-29 U/μl and at a high concentration (HC) of 250 U/μl. Both preparations are free of detectable protease and have specific activity > 1 × 106 U/mg protein. The > 99% purity grade is tested for endotoxins and contains < 0.25 EU/1000 units. The product is supplied as a 0.2 μm filtered solution in 50% glycerol. Store at -20°C.
Total endotoxin : < 0.25 EU/1,000 units. Purity : > 90% by SDS-PAGE.
Application
Used for the removal of nucleic acid from protein samples.
Biochem/physiol Actions
Digests native or heat-denatured DNA and RNA.
Other Notes
One unit is defined as the amount of enzyme that causes a A260 of 1.0 in 30 minutes, which corresponds to complete digestion of 37 μg DNA.
Legal Information
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
POPCULTURE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling
Ta strona może zawierać tekst przetłumaczony maszynowo.
Still not finding the right product?
Explore all of our products under Benzonase® Nuclease HC, Purity > 90%
Klasa składowania
10 - Combustible liquids
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
-
Hello, what is the suggested dilution of 71205-3? I have used 1:5000 dilution in past but I don't know if the stock concentration of this reagent has changed or not. Thank you!
1 answer-
Helpful?
-