71404-M

Rosetta 2(DE3)pLacI Competent Cells - Novagen

Escherichia coli, rod shaped

• UNSPSC Code: 41106202
• NACRES: NA.81
• Biological source: Escherichia coli
• Growth mode: adherent or suspension
• Morphology: rod shaped
• Manufacturer/tradename: Novagen^®

Właściwości

• Nazwa produktu: Rosetta 2(DE3)pLacI Competent Cells - Novagen, Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.
• biological source: Escherichia coli
• Quality Segment: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable, molecular cloning: suitable
• cell transformation: transformation efficiency: 2×10⁶ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Opis

General description

Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.

Genotype: F^–ompT hsdS_B (r_B^– m_B^–) gal dcm (DE3) pLacIRARE2 (Cam^R)





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Expression host strains for pETBlue and pTriEx vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7lac promoter in the absence of inducer. The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA.

Note that these strains are not recommended for use with pET, pACYCDuet, pCDF, pRSF, pCOLADuet or pETcoco vectors.

.Rosetta 2 strains are BL21 derivatives designed to alleviate codon bias when expressing heterologous proteins in E. coli. The cells carry a chloramphenicol-resistant plasmid with the tRNA genes that decode seven rare codons (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) to improve the yield of full-length proteins. The tRNA genes are driven by their native promoters. In Rosetta 2(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

Other Notes

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlRosetta 2(DE3)pLacI Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the “EMD Product”) requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids
• WGK: WGK 2