ABE1463

Anti-TopBP1 Antibody

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, WB
• Citations: -

Właściwości

• conjugate: unconjugated
• Quality Segment: 300
• antibody form: serum
• clone: polyclonal
• purified by: (unpurified)
• species reactivity: mouse, rabbit, human
• concentration: (Please refer to lot specific datasheet.)
• technique(s): immunocytochemistry: suitable, western blot: suitable
• UniProt accession no.: Q92547
• target post-translational modification: unmodified

Opis

General description

Recombinant protein corresponding to human TopBP1.

~180 kDa observed

Application

Western Blotting Analysis: A representative lot detected TopBP1 in U2OS and Saos-2 (Jeon, Y., et al. (2007). J. Biol. Chem. 282(20):14882-14890).
Western Blotting Analysis: A representative lot detected TopBP1 in mouse embryonic stem cells, early mouse embryos, and 3T3 cells (Jeon, Y., et al. (2011). J. Biol. Chem. 286(7):5414-5422).
Immunocytochemistry Analysis: A representative lot detected TopBP1 in 3T3, MEF, HS68, and IMR90 cells (Jeon, Y., et al. (2011). J. Biol. Chem. 286(7):5414-5422).

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Preparation Note

Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting in Saos-2 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected TopBP1 in 10 µg of Saos-2 cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• Co się dzieje?: WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable