ABE44-S
Anti-trimethyl Histone H3 (Lys27) Antibody, Trial Size
from rabbit, purified by affinity chromatography
Synonim(y):
H3K27me3, Histone H3 (trimethyl K27), H3 histone family, member T, Histone 3, H3, Histone cluster 3, H3, H3K27me3
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Informacje o tej pozycji
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human, mouse
Application:
DB, ICC, WB, inhibition assay
Citations:
3
biological source
rabbit
Quality Segment
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
technique(s)
dot blot: suitable, immunocytochemistry: suitable, inhibition assay: suitable (peptide), western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
trimethylation (Lys27)
Gene Information
human ... HIST1H3F(8968)
General description
Histone H3 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 lysine 27 methylation (H3K27) is associated with heterochromatin formation and transcriptional repression.
~ 17 kDa
Application
Anti-trimethyl Histone H3 (Lys27) Antibody is a rabbit polyclonal antibody for detection of Histone H3 trimethylated on lysine 27 (H3K73me). This purified Ab is specificity verified by dot blot (DB) & validated in WB, ICC, PIA. Small pack size is ideal for screening applications.
Biochem/physiol Actions
Broad species cross-reactivity expected.
Analysis Note
Evaluated by Western Blot in HeLa acid extract and recombinant Histone H3.
Western Blot Analysis: 0.1 µg/ml of this antibody detected histone H3 in 10 µg of HeLa acid extract .
Western Blot Analysis: 0.1 µg/ml of this antibody detected histone H3 in 10 µg of HeLa acid extract .
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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