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CBL434

Anti-c-Myc Tag Antibody

CHEMICON®, mouse monoclonal, 3C7

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Informacje o tej pozycji

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
3C7, monoclonal
Species reactivity:
mouse, human
Application:
ELISA, IP, WB
Citations:
6


Nazwa produktu

Anti-c-myc Antibody, clone 3C7, clone 3C7, Chemicon®, from mouse

biological source

mouse

Quality Segment

antibody form

purified antibody

antibody product type

primary antibodies

clone

3C7, monoclonal

species reactivity

mouse, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MYC(4609)
mouse ... Myc(17869)

Immunogen

Epitope: a.a. 171-188
Synthetic peptide: Cys-Ser-Thr-Ser-Ser-Leu-Tyr-Leu-Gln-Asp-Leu-Ser-Ala-Ala-Ala-Ser-Glu-Cys selected from the human c-myc sequence (amino acids (171-188). The peptide was conjugated to keyhole limpet haemocyanin.

Application

Detect c-myc also known as Proto-oncogene c-Myc with Anti-c-myc Antibody, clone 3C7 (Mouse Monoclonal Antibody), that has been demonstrated to work in ELISA, IP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Western blotting

Immunoprecipitation

Immunoassay detection of c-myc

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

In immunoblotting this antibody detects one major band at 62-64 kDa which can be blocked by pre-incubation with the immunizing peptide. In immunoprecipitation the antibody detects a 62 kDa band from cell lysates.

KNOWN SPECIES CROSS REACTIVITY: This antibody is known to cross react with mouse c-myc

Physical form

Format: Purified
The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Preparation Note

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ta pozycja
M4439M5546C3956
description

clone 3C7, Chemicon®, from mouse

description

clone 9E10, purified from hybridoma cell culture

description

clone 9E10, ascites fluid

description

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

species reactivity

mouse, human

species reactivity

human

species reactivity

human

species reactivity

human

clone

3C7, monoclonal

clone

9E10, monoclonal

clone

9E10, monoclonal

clone

polyclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

antibody form

purified antibody

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

affinity isolated antibody

Gene Information

human ... MYC(4609)
mouse ... Myc(17869)

Gene Information

human ... MYC(4609)

Gene Information

human ... MYC(4609)

Gene Information

human ... MYC(4609)

technique(s)

western blot: suitable, ELISA: suitable, immunoprecipitation (IP): suitable

technique(s)

microarray: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, immunocytochemistry: suitable, western blot: 1:5000 using c-myc tagged fusion protein

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: 1:100 using c-myc tagged fusion protein, indirect ELISA: suitable, immunocytochemistry: suitable

technique(s)

western blot: 0.5-1 μg/mL using detects c-Myc fusion proteins in cell extracts from transfected cultures as well as bacterial lysates., immunoprecipitation (IP): 1-2 μg using immunoprecipitates a c-Myc fusion protein from transfected mammalian cell lysates or bacterial extracts, indirect immunofluorescence: 5-10 μg/mL using detects c-Myc fusion proteins in methanol-acetone fixed transiently transfected cells, immunocytochemistry: 5-10 μg/mL, microarray: suitable


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