CC130

Human Vitronectin

recombinant, expressed in E. coli, liquid, 0.5 mg/mL, used as an effective xeno-free alternative to matrigel, suitable for human ES and iPS cell culture, PLURISTEM^®

• Synonim(y): S-protein, Serum-spreading factor, V75
• Kod UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

Właściwości

• Nazwa produktu: PluriSTEM-XF^® Recombinant Vitronectin, Human pluripotent stem cell culture.
• pochodzenie biologiczne: Escherichia coli; human
• Poziom jakości: 100
• Próba: ≥92% (SDS-PAGE)
• Formularz: liquid
• producent / nazwa handlowa: PluriSTEM^®
• stężenie: 0.5 mg/mL
• metody: cell attachment: suitable; cell culture | mammalian: suitable; cell culture | stem cell: suitable
• moc wejściowa: sample type: human embryonic stem cell(s)
• numer dostępu UniProt: P04004
• Warunki transportu: dry ice

Opis

Opis ogólny

PluriSTEM Recombinant Vitronectin is an effective xeno-free alternative to matrigel and has been validated to successully culture human ES and iPS cells using PluriSTEM Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.

PluriSTEM Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.

Zastosowanie

Research Category
Stem Cell Research

Cell Structure

ECM & Adhesion

Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.

Postać fizyczna

Product is supplied in a 20 mM pH 8.0 Tris-HCl buffer with a proprietary formulation of sucrose, EDTA, arginine, and DTT.

Uwaga dotycząca przygotowania

Store at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles. Product has been 0.2 μm filtered.

Komentarz do analizy

Each lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).

Informacje prawne

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable