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Merck

IP05

Protein G Plus/Protein A Agarose Suspension

Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Synonim(y):

Affinity resin, Protein G/Agarose

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1.5 ML

757,00 zł

757,00 zł

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Informacje o tej pozycji

NACRES:
NA.56
UNSPSC Code:
41116133

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form

slurry (Liquid)

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

technique(s)

protein purification: suitable

suitability

suitable for microbiology

shipped in

wet ice

storage temp.

2-8°C

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Ta pozycja
IP04IP10P1925
Protein G Plus/Protein A Agarose Suspension Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Millipore

IP05

Protein G Plus/Protein A Agarose Suspension

Protein G Plus-Agarose Suspension Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.

Millipore

IP04

Protein G Plus-Agarose Suspension

Protein G Plus/Protein A-Agarose A mixture of Protein G PLUS and Protein A covalently conjugated to agarose. Useful for purification of IgG from biological fluids.

Millipore

IP10

Protein G Plus/Protein A-Agarose

Protein A–Agarose macrobeads aqueous suspension

Millipore

P1925

Protein A–Agarose macrobeads

technique(s)

protein purification: suitable

technique(s)

immunoprecipitation (IP): suitable

technique(s)

protein purification: suitable

technique(s)

-

form

slurry (Liquid)

form

slurry (Liquid)

form

slurry (Liquid)

form

aqueous suspension

suitability

suitable for microbiology

suitability

suitable for immunoprecipitation

suitability

suitable for microbiology

suitability

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

contains

≤0.1% sodium azide as preservative

contains

≤0.1% sodium azide as preservative

contains

≤0.1% sodium azide as preservative

contains

-

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

General description

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Application

Immunoprecipitation (see comments)

Physical form

33% slurry in PBS.

Other Notes

Agarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)
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Klasa składowania

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Paz J Luncsford et al.
DNA repair, 12(12), 1043-1052 (2013-11-12)
MutY homologue (MYH) is a DNA glycosylase which excises adenine paired with the oxidative lesion 7,8-dihydro-8-oxoguanine (8-oxoG, or G(o)) during base excision repair (BER). Base excision by MYH results in an apurinic/apyrimidinic (AP) site in the DNA where the DNA
Ji Hyun Shin et al.
Autophagy, 15(9), 1495-1505 (2019-03-02)
Several studies have shown that dysfunction of macroautophagy/autophagy is associated with many human diseases, including neurodegenerative disease and cancer. To explore the molecular mechanisms of autophagy, we performed a cell-based functional screening with SH-SY5Y cells stably expressing GFP-LC3, using an
Yi Guan et al.
Journal of the American Society of Nephrology : JASN, 28(8), 2337-2352 (2017-03-02)
The rapid growth of an aging population creates challenges regarding age-related diseases, including AKI, for which both the prevalence and death rate increase with age. The molecular mechanism by which the aged kidney becomes more susceptible to acute injury has
Lixuan Zhan et al.
Journal of neuroinflammation, 18(1), 97-97 (2021-04-22)
Our previous study indicated that hypoxic preconditioning reduced receptor interacting protein (RIP) 3-mediated necroptotic neuronal death in hippocampal CA1 of adult rats after transient global cerebral ischemia (tGCI). Although mixed lineage kinase domain-like (MLKL) has emerged as a crucial molecule
Fariborz Mortazavi et al.
Clinical & experimental metastasis, 28(4), 391-404 (2011-02-22)
As a member of adherens junction, p120-catenin (p120ctn) plays a major role in cell adhesions through stabilization of E-cadherin. p120ctn is transcriptionally down-regulated in non-small cell lung cancer (NSCLC), although the molecular mechanisms underlying p120ctn repression are incompletely defined. Here
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Agarose Beads for Immunoprecipitation and Antibody Purification

We manufacture IP and antibody purification agarose beads that purify immunoglobulins and IgG fractions. Our kits also provide a total antibody purification process

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SKUNUMER GTIN
IP05-1.5ML04055977220223

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