This material has not been tested for suitability in the application for IHC-F.
MAB360
Anti-Glial Fibrillary Acidic Protein Antibody
CHEMICON®, mouse monoclonal, GA5
Synonim(y):
GFAP
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
25 μL | Sprawdź dostępność w koszyku | 836,00 zł |
100 μL | Sprawdź dostępność w koszyku | 1800,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
GA5, monoclonal
Species reactivity:
rat, human, mouse, rabbit, chicken, pig, bovine
Application:
ICC, IHC (p), WB
Citations:
1135
Pomoc techniczna
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Pozwól nam pomócNazwa produktu
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5, ascites fluid, clone GA5, Chemicon®
biological source
mouse
Quality Segment
antibody form
ascites fluid
antibody product type
primary antibodies
clone
GA5, monoclonal
species reactivity
rat, human, mouse, rabbit, chicken, pig, bovine
packaging
antibody small pack of 25 μL
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GFAP(2670)
General description
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
~ 51 kDa
Immunogen
Purified GFAP from porcine spinal cord.
Application
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB with more than 65 product citations.
Immunocytochemistry:
A previous lot was used on cultured mammalian cells.
Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.
Immunoblotting: A 1:1000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user
A previous lot was used on cultured mammalian cells.
Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.
Immunoblotting: A 1:1000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user
Biochem/physiol Actions
Glial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin.
Reactivity with other species has not been determined.
Physical form
Unpurified mouse monoclonal IgG1 liquid in buffer containing 15 mM sodium azide.
Analysis Note
Control
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
Routinely evaluated by Western Blot on Mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.
Other Notes
Replaces: 04-1031; 04-1062
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Ta pozycja | |||
|---|---|---|---|
| description ascites fluid, clone GA5, Chemicon® | description clone GA5, Chemicon®, from mouse | description clone GA5, Chemicon®, from mouse | description IgG fraction of antiserum, buffered aqueous solution |
| antibody form ascites fluid | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form IgG fraction of antiserum |
| species reactivity rat, human, mouse, rabbit, chicken, pig, bovine | species reactivity chicken, pig, rat, bovine, mouse, rabbit, human | species reactivity human, chicken, pig, rat | species reactivity rat, human |
| clone GA5, monoclonal | clone GA5, monoclonal | clone GA5, monoclonal | clone polyclonal |
| Gene Information human ... GFAP(2670) | Gene Information human ... GFAP(2670) | Gene Information human ... GFAP(2670) | Gene Information human ... GFAP(2670) |
| biological source mouse | biological source mouse | biological source mouse | biological source rabbit |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
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Klasa składowania
10 - Combustible liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Can this antibody be used in IHC frozen(IHC-F) rather than IHC paraffin(IHC-P)?
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