As you can see in the example image, this just stains every nucleus. That's not what you expect in liver, and that's not what you expect in pretty much any tissue. Not sure why we bought this one, but we definitely won't be buying it again.
MABF81
Anti-ROR gamma T Antibody, clone 6F3.1
clone 6F3.1, from mouse
Synonim(y):
Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3, Retinoid-related orphan receptor-gamma
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
100 μg | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 1880,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
6F3.1, monoclonal
Application:
IHC, WB
Citations:
21
Pomoc techniczna
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Pozwól nam pomócbiological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6F3.1, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable, western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RORC (6097)
General description
Retinoid-related orphan receptor-gamma t (RORγT) is one of two RORγ isoforms belonging to the nuclear hormone receptor family and contains a single nuclear receptor DNA-binding domain. RORγT is a negative regulator of TNFDF6/FASL expression and T-cell production of IL2. The upregulation of RORγT is a requirement for TH17 cell differentiation. Inhibition of RORγT is induced by TGF-β’s interaction with Foxp3. Expression is observed mainly in immature thymocytes, and, during fetal development, is found exclusively in lymphoid-inducer (LTi) cells.
~55 kDa observed. Two isoforms at ~55 kDa and ~58 kDa may be observed in some cell lysates.
Immunogen
GST-tagged recombinant protein corresponding to human ROR gamma T.
Application
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected ROR gamma T in normal human liver tissues.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Inflammation & Autoimmune Mechanisms
This Anti-ROR gamma T Antibody, clone 6F3.1 is validated for use in WB, IH for the detection of ROR gamma T.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
T47D cell lysate
T47D cell lysate
Evaluted in Western Blot in T47D cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected ROR gamma T in 10 µg of T47D cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected ROR gamma T in 10 µg of T47D cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ta pozycja | |||
|---|---|---|---|
| description clone 6F3.1, from mouse | description recombinant, expressed in HEK 293 cells | description recombinant, expressed in HEK 293 cells | description Chemicon®, from rabbit |
| antibody form purified immunoglobulin | antibody form purified antibody | antibody form purified antibody | antibody form affinity purified immunoglobulin |
| clone 6F3.1, monoclonal | clone 6F3.1, recombinant monoclonal | clone 1D13, recombinant monoclonal | clone polyclonal |
| Gene Information human ... RORC (6097) | Gene Information - | Gene Information - | Gene Information human ... RORB(6096) |
| species reactivity human | species reactivity human | species reactivity mouse, human | species reactivity human |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| biological source mouse | biological source mouse | biological source rabbit | biological source rabbit |
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Klasa składowania
12 - Non Combustible Liquids
WGK
WGK 1
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