The dissection requirement is that the head, liver, and visceral tissues be taken. Unfortunately, other than the source of the dissected tissues, the specific origin is not reported.
PMEF-HL
EmbryoMax® Primary Mouse Embryonic Fibroblasts
Mouse
Synonim(y):
Hygro MEF Feeder Cells, Hygro Mouse Feeder Cells, Hygro-MEFs, Hygromycin Restistant PMEFs, MEF Feeder Cells, hygro PMEFs, hygro MEFs, MEFs, Mouse Feeder Cells
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
5 vials | Przewidywany termin wysyłki25 września 2026zKuehne + Nagel Sp. z o.o. | 2060,00 zł |
Informacje o tej pozycji
UNSPSC Code:
41106509
NACRES:
NA.75
eCl@ss:
32011203
Biological source:
mouse
Manufacturer/tradename:
Specialty Media, EmbryoMax®
2060,00 zł
Przewidywany termin wysyłki25 września 2026Szczegóły
Pomoc techniczna
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Pozwól nam pomócNazwa produktu
EmbryoMax® Primary Mouse Embryonic Fibroblasts, PMEF, Hygro Resistant, Strain C57/BL6, Not Treated, Passage 3
biological source
mouse
Quality Segment
manufacturer/tradename
Specialty Media, EmbryoMax®
technique(s)
cell culture | stem cell: suitable
input
sample type: mouse embryonic stem cell(s)
sample type primary embryotic fibroblasts (PMEFs)
sample type induced pluripotent stem cell(s)
shipped in
liquid nitrogen
storage temp.
-140 to -196°C
General description
Primary Mouse Embryo Fibroblasts, hygromycin resistant, strain C57/BL6, are not Mytomycin C treated and serve as a feeder layer for both mouse and human ES/IPS cells. MEF cells are resistant to hygromycin. The cells are provided at passage 3 and can be further expanded before freezing for later use.
Plating MEF Feeder Cells
Procedure:
1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.
Plating MEF Feeder Cells
Procedure:
1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
appearance. Gelatinized plates may be used for 12–14 days.
The EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
Biochem/physiol Actions
Primary Mouse Embryo Fibroblasts
Packaging
5-6x106 ea
Physical form
The cells are provided frozen into individual vials, containing approximately 5-6 x 106 fibroblasts per vial, and are sold as a pack of 5 vials and supplied at passage 3.
Preparation Note
After receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
Legal Information
EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany
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Klasa składowania
12 - Non Combustible Liquids
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does not flash
flash_point_c
does not flash
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Would it be possible to get a more precise information on the dissection of these fibroblasts -- i.e. were the head, vertebral column, dorsal root ganglia, and visceral organs removed? Or were these fibroblasts obtained from limbs only?
1 answer-
Helpful?
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