SCC007

ReNcell CX Human Neural Progenitor Cell Line

ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells.

• Synonim(y): Human NSC Line, Human Neural Stem Cell Line, Human Neural Stem Cells
• UNSPSC Code: 41106509
• NACRES: NA.75
• eCl@ss: 32011203
• Biological source: human

Właściwości

• biological source: human
• Quality Segment: 100
• manufacturer/tradename: Chemicon^®, ReNcell
• technique(s): cell culture | stem cell: suitable
• input: sample type neural stem cell(s)
• shipped in: liquid nitrogen
• storage temp.: -140 to -196°C

Opis

General description

Product Source: Human cortical brain tissue

ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell CX was derived from the cortical region of human fetal brain. Immortalized by retroviral transduction with the c-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell CX retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). ReNcell CX was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. ReNcell CX may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ReNcell CX cells have been obtained in a legal and ethical manner, compliant with current local informed consent procedures.

ReNcell Now Used in a 3D Cell Model of Alzheimer′s Disease!
Tanzi, R and Kim, DY et. al. A three-dimensional human neural cell culture model of Alzheimer′s disease. Nature. 2014 Nov 13;515(7526):274-8.
(Published in Nature)

Application

Research Category
Stem Cell Research

Biochem/physiol Actions

Neural Stem Cells

Preparation Note

When stored at the recommended storage conditions (liquid nitrogen), ReNcell CX cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.

Analysis Note

Each lot of ReNcell CX cells has been validated for high level of expression of Nestin and Sox 2 and for their self-renewal and multi-lineage differentiation capacities (please refer to datasheet figures). Cells also display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma. Maternal blood has been screened for HIV, HTLV, Hepatitis B and C.

Other Notes

> 1x10⁶ viable ReNcell CX cells

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash