SCC064

LX-2 Human Hepatic Stellate Cell Line

Human

• Synonim(y): HSC, Perisinusoidal cells, Ito cells, Hepatic lipocytes, Hepatic pericytes
• UNSPSC Code: 41106514
• NACRES: NA.75
• eCl@ss: 32011203
• Biological source: human

Właściwości

• Nazwa produktu: LX-2 Human Hepatic Stellate Cell Line, a highly suitable model of human hepatic fibrosis
• biological source: human
• Quality Segment: 100
• technique(s): cell culture | stem cell: suitable, cell culture: suitable using | mammalian
• shipped in: liquid nitrogen
• storage temp.: -140 to -196°C

Opis

General description

Listen to an Audio Overview

Hepatic stellate cells are a major cell type responsible for liver fibrosis following their activation into fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. There is an increasing need for renewable cell culture models that faithfully recapitulate their in vivo phenotype, particularly for human studies.

LX-2 was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passaged cells in low serum media conditions.

Immortalized LX-2 was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous. These cell lines have been extensively characterized and retain key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them highly suitable for culture based studies of human hepatic fibrosis.


MilliporeSigma is the exclusive license holder of the LX-2 Human Hepatic Stellate Cell Line from Mount Sinai.

Application

Research Category
Infectious Diseases

Inflammation & Immunology

Stem Cell Research

Preparation Note

LX-2 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality. LX-2 cells have been successfully expanded past passage 50 in culture.

Analysis Note

• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested by PCR and are negative for Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negatrive for mycoplasma contamination.

Other Notes

1) ≥1X10⁶ viable LX-2 Human Hepatic Stellate Cells: (Catalog No. SCC064). Store in liquid nitrogen.

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids
• WGK: WGK 1