Spontaneous contraction, such as beating, of HL-1 cells may not be observable by bright-field microscopic inspection due to its subtlety. The beating phenotype can be observed using a fluorescent calcium flux dye, Fluo-8®.
SCC065
HL-1 Cardiac Muscle Cell Line
Mouse
Synonim(y):
HL-1 Cardiomyocyte Cell Line, HL-1 Atrial Muscle Cell Line
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 vial | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | Prześlij zapytanie |
Informacje o tej pozycji
UNSPSC Code:
41106514
NACRES:
NA.81
eCl@ss:
32011203
Biological source:
mouse
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Pozwól nam pomócNazwa produktu
HL-1 Cardiac Muscle Cell Line, HL-1 Cardiac Muscle Cell Line has been extensively characterized and is a valuable model system to address questions of cardiac biology at the cellular & molecular levels.
biological source
mouse
Quality Segment
technique(s)
cell based assay: suitable, cell culture | mammalian: suitable
General description
Listen to an Audio Overview
HL-1 Cardiac Muscle Cell Line is an immortalized mouse cardiomyocyte cell line able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 can be serially passaged without losing its differentiated cardiac myocyte phenotype, including morphological, biochemical, and electrophysiological properties. HL-1 cells have been used to study normal cardiomyocyte function with regard to signaling, electrical, metabolic, and transcriptional regulation as well as to address pathological conditions such as hypoxia, hyperglycemia-hyperinsulinemia, apoptosis, and ischemia-reperfusion.
The HL-1 line was derived from AT-1 subcutaneous tumor excised from an adult female C57BL/6J mouse. The parental AT-1 line was originally derived from an atrial tumor growing in a transgenic mouse in which expression of the SV40 large T-antigen was targeted to atrial cardiomyocytes via the atrial natriuretic factor (ANF) promoter.
HL-1 Cardiac Muscle Cell Line is an immortalized mouse cardiomyocyte cell line able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 can be serially passaged without losing its differentiated cardiac myocyte phenotype, including morphological, biochemical, and electrophysiological properties. HL-1 cells have been used to study normal cardiomyocyte function with regard to signaling, electrical, metabolic, and transcriptional regulation as well as to address pathological conditions such as hypoxia, hyperglycemia-hyperinsulinemia, apoptosis, and ischemia-reperfusion.
The HL-1 line was derived from AT-1 subcutaneous tumor excised from an adult female C57BL/6J mouse. The parental AT-1 line was originally derived from an atrial tumor growing in a transgenic mouse in which expression of the SV40 large T-antigen was targeted to atrial cardiomyocytes via the atrial natriuretic factor (ANF) promoter.
Application
Research Category
Cardiac
Cardiovascular Disease
Toxicity
Cardiac
Cardiovascular Disease
Toxicity
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.
Biochem/physiol Actions
Cardiomyocyte Cells
Preparation Note
HL-1 Cardiac Muscle Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Analysis Note
• Each vial contains ≥ 1X106 viable cells.
• Cells tested negative for infectious diseases by the Mouse Essential CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Functional Assay: Fluo-8 calcium dye fluorescent based assay for detecting changes in intracellular calcium
• Cells tested negative for infectious diseases by the Mouse Essential CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Functional Assay: Fluo-8 calcium dye fluorescent based assay for detecting changes in intracellular calcium
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
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| description HL-1 Cardiac Muscle Cell Line has been extensively characterized and is a valuable model system to address questions of cardiac biology at the cellular & molecular levels. | description HMC-1.1 human mast cell line is a variant subline of the HMC-1 cell line and possesses the V560G KIT mutation. | description HMC-1.2 human mast cell line is a variant subline of the HMC-1 cell line and possesses both the V560G and D816V KIT mutations. | description The N27 rat dopaminergic neural cell line has been used as a dopaminergic neuron model for in vitro and in vivo studies. |
| biological source mouse | biological source human | biological source human | biological source rat |
| technique(s) cell based assay: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | stem cell: suitable, cell culture: suitable using | mammalian |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Klasa składowania
10 - Combustible liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Hello, Are these HL1 cells the type that do not beat in culture? Please answer, yes or no.
1 answer-
Helpful?
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Hello, Are these HL1 cells the type that do not beat in culture?
1 answer-
HL-1 Cardiac Muscle Cell Line is an immortalized mouse cardiomyocyte cell line able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 can be serially passaged without losing its differentiated cardiac myocyte phenotype, including morphological, biochemical, and electrophysiological properties. HL-1 cells have been used to study normal cardiomyocyte function with regard to signaling, electrical, metabolic, and transcriptional regulation as well as to address pathological conditions such as hypoxia, hyperglycemia-hyperinsulinemia, apoptosis, and ischemia-reperfusion.
The HL-1 line was derived from AT-1 subcutaneous tumor excised from an adult female C57BL/6J mouse. The parental AT-1 line was originally derived from an atrial tumor growing in a transgenic mouse in which expression of the SV40 large T-antigen was targeted to atrial cardiomyocytes via the atrial natriuretic factor (ANF) promoter.
Helpful?
-