SCC231

YUMMER1.G1A Mouse Melanoma Cell Line

• Synonim(y): YUMMER1.G1A Mouse Melanoma Cell Line, Yale University Mouse Melanoma 1.G1A cell line, Yale University Mouse Melanoma cell line
• UNSPSC Code: 41106514
• NACRES: NA.51
• Biological source: mouse
• Manufacturer/tradename: Millipore

Właściwości

• biological source: mouse
• Quality Segment: 100
• packaging: vial of ≥1X10⁶ vial (viable cells per vial)
• manufacturer/tradename: Millipore
• technique(s): cell culture | mammalian: suitable
• shipped in: liquid nitrogen
• storage temp.: −196°C

Opis

General description

As immune-based therapies in cancer models become more popular and applicable, a further need for immune-competent models has been presented. A wide variety of genetically engineered mouse models have been created to express vital melanoma genotypes. The variety in these models have allowed the study of genetic alterations that are relevant to human melanoma biology. While it is quite useful to have mouse models with genetically defined human melanoma characteristics, it is limited by complex mouse colony maintenance. The YUMMER1.G1A cell line was produced to be more experimentally relevant. The cell line was derived by backcrossing important alleles into C57BL/6J mice. The mice were interbred to produce human-relevant genetically defined mouse models that could then be used to generate cell lines. Melanomas were induced by applying 4-hydroxytamoxifen to induce Cre-lox allele recombination. Cells were then mechanically and enzymatically dissociated from the removed tumor. The melanoma lines generated from this procedure retained important human melanoma characteristics useful for studying disease progression.The YUMMER1.G1A cell line is derived from the YUMM1.G1 cell line (SCC230) which is sourced from a Mc1re/e female mouse model driven by Braf activation, Pten inactivation, and Cdkn2a inactivation.1 The YUMM1.G1 cells were further subcloned into the YUMMER1.G1A cell line by undergoing UVB radiation induced mutagenesis, resulting in high mutational burden. The cell line is positive for the common melanoma markers, MelanA and PMEL. The cells are syngeneic with C57BL/6J mice and are very tumorigenic in vivo.References:1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.

Application

• Each vial contains > 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.

Features and Benefits

YUMMER1.G1A cell line is derived from a Mc1re/e female mouse model driven by Braf activation, Pten inactivation, and Cdkn2a inactivation. The cells were further subcloned into theYUMMER1.G1A cell line by undergoing UVB radiation induced mutagenesis.

Preparation Note

YUMMER1.G1A cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Analysis Note

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids