SCC733

3dGRO^® Colorectal Organoids (POP.141.G2)

Adenocarcinoma, metastatic (liver), Colon, Age: 57, Male, Mutation: Not Available

• Synonim(y): organoid, PDO, cancer organoid, tumor organoid, 3d cell culture, colorectal cancer, colorectal organoids
• NACRES: NA.21
• UNSPSC Code: 41106514

Właściwości

• Quality Segment: 100
• biological source: human colon
• packaging: vial of ≥1500 organoids/vial vials
• manufacturer/tradename: Millipore
• gender: male
• technique(s): cell culture: suitable
• application(s): 3D cell culture
• control: colon
• relevant disease(s): adenocardinoma (metastatic liver)
• shipped in: liquid nitrogen
• storage temp.: −196°C

Opis

General description

Colorectal cancer accounts for roughly 10% of all cancer cases worldwide with more than half of all patients with CRC developing metastatic disease leading to death. Recently, tissue derived organoids have emerged as a more predictive 3 dimensional cell culture model of disease. 3D organoid cultures conserve the original genetic and phenotypic characteristics of the primary tissue allowing for their application in many research fields included drug development, personalized medicine and potential therapeutics. In vitro cultured tumor organoids have also been shown to predict patient response to chemotherapeutics. PDOs derived from colorectal cancer (CRC organoids) have been used for cell modeling and to investigate the function of cancer related driver gene mutations including APC, TP53, KRAS, BRAF, PIK3CA etc.

We are now offering a comprehensive biobank of highly characterized tissue-derived human gastrointestinal organoids from normal and diseased patients. The intestinal organoid biobank contains over 50 highly characterized intestinal organoids from both normal and diseased patients derived from multiple regions of the digestive systems including small intestine (duodenum, ileum), stomach, rectum and colon.

Cell Line Characteristics

• Sex: Male
• Age: 57 Years
• Organ: Colon (Xenograph from Primary Tumor, Liver Metastasis)
• Disease: Adenocarcinoma (KRAS Mutation: G12D)

Application

• Viability: >=1500 viable organoids/vial
• Organoid Growth: Pass
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Mycoplasma Contamination: Negative
• STR Profile: Pass

Features and Benefits

Cryopreserved patient derived colorectal cancer organoids (PDOs)

Preparation Note

Store in liquid nitrogen. The organoids can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Legal Information

3dGRO^® is a trademark of Merck KGaA, Darmstadt, Germany.
3dGRO^® organoids were derived utilizing HUB Organoid Technology.

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/pdouse. This product may not be re-engineered or copied; used to make derivatives or modifications; used to develop, test, or manufacture a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact organoids@milliporesigma.com.

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• WGK: WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable