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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
100 mL | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 219,00 zł |
Informacje o tej pozycji
Quality Segment
form
liquid
manufacturer/tradename
Chemicon®
technique(s)
cell culture | stem cell: suitable
input
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
shipped in
dry ice
storage temp.
-10 to -25°C
General description
Application
1. Thaw Accutase® to room temperature.
2. Wash plate, flask or beads with sterile PBS.
3. Add Accutase to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area.
4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).
5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.
Physical form
1X Accutase® enzymes in Dulbecco′s PBS containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red.
Preparation Note
Legal Information
1 of 1
Ta pozycja | |||
|---|---|---|---|
| description The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium. | description AccutaseXL is a lyophilized form of Accutase with extended stability used for the routine detachment of cells from standard tissue culture plasticware | description - | description A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue. |
| Quality Level 100 | Quality Level 300 | Quality Level 100 | Quality Level 300 |
| shipped in dry ice | shipped in dry ice | shipped in ambient | shipped in dry ice |
| storage temp. -10 to -25°C | storage temp. -10 to -25°C | storage temp. 15-25°C | storage temp. -10 to -25°C |
| technique(s) cell culture | stem cell: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | stem cell: suitable | technique(s) - |
| manufacturer/tradename Chemicon® | manufacturer/tradename Chemicon® | manufacturer/tradename - | manufacturer/tradename Chemicon® |
| input sample type: mouse embryonic stem cell(s) | input - | input sample type induced pluripotent stem cell(s) | input sample type cardiac stem cell(s) |
Klasa składowania
12 - Non Combustible Liquids
WGK
WGK 1
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