If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
Przejdź do
A4718
Agarose
Molecular Biology
Synonim(y):
3,6-Anhydro-α-L-galacto-β-D-galactan
Wybierz wielkość
| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
25 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1100,00 zł |
100 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2370,00 zł |
Informacje o tej pozycji
1100,00 zł
biological source
algae (marine)
Quality Segment
grade
Molecular Biology
form
powder
technique(s)
electrophoresis: suitable
mp
≤75 °C (3% gel)
transition temp
gel point ≤35 °C (3% gel)
foreign activity
DNase, RNase, NICKase, none detected
storage temp.
room temp
SMILES string
O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
General description
Application
Low ethidium bromide and SYBR Green background staining
Analysis Note
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Other Notes
1 of 1
Ta pozycja | |||
|---|---|---|---|
| description Molecular Biology | description Ultra-low Gelling Temperature | description Type I-A, low EEO | description Type I, Molecular Biology |
| form powder | form powder | form powder | form - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| grade Molecular Biology | grade - | grade - | grade Molecular Biology |
| technique(s) electrophoresis: suitable | technique(s) - | technique(s) - | technique(s) - |
| foreign activity DNase, RNase, NICKase, none detected | foreign activity - | foreign activity - | foreign activity - |
| biological source algae (marine) | biological source algae (marine) | biological source - | biological source - |
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Klasa składowania
11 - Combustible Solids
WGK
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
Eyeshields, Gloves, type N95 (US)
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How can I determine the shelf life / expiration / retest date of this product?
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Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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Is this agarose high-resolution, and suitable for PCR applications?
1 answer-
The High Resolution Agarose, product number A4718, is an intermediate-melting agarose that offers approximately double the resolution capability compared to standard agarose. It is capable of resolving PCR products and DNA fragments ranging from 200-800 bp that differ in size by as little as 2%. This makes it ideal for separating small DNA fragments, such as PCR products, with very small size differences, offering a resolution comparable to that achieved with polyacrylamide gels.
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