B4380

Bromobimane

≥97% purity, powder

• Synonim(y): Monobromobimane
• Wzór empiryczny (zapis Hilla): C₁₀H₁₁BrN₂O₂
• Numer CAS: 71418-44-5
• Masa cząsteczkowa: 271.11
• NACRES: NA.47
• PubChem Substance ID: 24891769
• UNSPSC Code: 12171500
• MDL number: MFCD00036951
• Beilstein/REAXYS Number: 4430959

Właściwości

• Nazwa produktu: Bromobimane, ≥97% (HPLC)
• Quality Segment: 200
• assay: ≥97% (HPLC)
• form: powder
• color: yellow
• mp: 161 °C
• solubility: acetonitrile: 20 mg/mL
• ε (extinction coefficient): 4.6-5.1 at 396-398 nm in H₂O
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: CC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O
• InChI: 1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3
• InChI key: AHEWZZJEDQVLOP-UHFFFAOYSA-N

Opis

General description

Bromobimane is also known as monobromobimane. It is also a known probe for thiols and is a fluorescent reagent activated upon a photolysis reaction.

Application

Bromobimane is used for the determination of thiols by the HPLC method. It is suitable as a pre-column derivatization agent for fluorometric determination of 2,3-dimercaptopropane-1-sulfonic acid and other dithiols. Bromobimane has been used as a fluorescent label in studying oligomycin-sensitive ATPase from beef heart mitochondria.
Bromobimane has been used for the quantitative measurement of free hydrogen sulfide in vivo and in vitro. It has been used for the labeling of proteins containing thiol groups.

Biochem/physiol Actions

Bromobimane in solution reacts with small thiol groups (e.g., GSH) and with reactive protein thiol groups (e.g., hemoglobin). The reaction of Bromobimane with thiols is of second-order and dependent on pH and upon reacting with thiolate, it activates the water-soluble fluorescent product for detection.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• WGK: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): Eyeshields, Gloves, type N95 (US)