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D9564

DAPI, dilactate

≥98% purity (HPLC), powder

1 of 1 reviewers received a sample product or took part in a promotion

Synonim(y):

4′,6-Diamidino-2-phenyindole, dilactate

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Rozmiar/SKUDostępnośćCena netto
10 mg
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1160,00 zł

Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C16H15N5 · 2C3H6O3
Masa cząsteczkowa:
457.48
MDL number:
MFCD00467366
UNSPSC Code:
12171500
PubChem Substance ID:
329798905
NACRES:
NA.47

1160,00 zł

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Nazwa produktu

DAPI, dilactate, ≥98% (HPLC)

Quality Segment

assay

≥98% (HPLC)

form

powder

technique(s)

titration: suitable

color

yellow

solubility

H2O: 20 mg/mL

ε (extinction coefficient)

28.00-32.50 at 345-351 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

CC(O)C(O)=O.CC(O)C(O)=O.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

InChI=1S/C16H15N5.C3H6O3/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;1-2(4)3(5)6/h1-8,21H,(H3,17,18)(H3,19,20);2,4H,1H3,(H,5,6)

InChI key

LCAUPANIXBMNIA-UHFFFAOYSA-N

General description

DAPI is an indolenine dye. It carries two amidine components, one on a pendant phenyl ring and another directly on the indole moiety.

Application

DAPI, dilactate has been used for the nuclear staining in cells. DAPI is a common flow cytometry stain that is widely used as a DNA and nuclear stain. Additionally, it is used as a counterstain following in situ hybridization and other immunofluorescence procedures.

Biochem/physiol Actions

A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.
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Ta pozycja
268298D954210236276001
DAPI, dilactate ≥98% (HPLC)

Sigma-Aldrich

D9564

DAPI, dilactate

DAPI, Dihydrochloride Cell-permeable DNA-binding dye.

Sigma-Aldrich

268298

DAPI, Dihydrochloride

DAPI for nucleic acid staining

Sigma-Aldrich

D9542

DAPI

DAPI 4′,6-Diamidine-2′-phenylindole dihydrochloride

Roche

10236276001

DAPI

description

≥98% (HPLC)

description

Cell-permeable DNA-binding dye.

description

for nucleic acid staining

description

4′,6-Diamidine-2′-phenylindole dihydrochloride

technique(s)

titration: suitable

technique(s)

-

technique(s)

transfection: suitable

technique(s)

-

color

yellow

color

yellow

color

-

color

-

form

powder

form

solid

form

powder

form

powder

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

application(s)

-

solubility

H2O: 20 mg/mL

solubility

methanol: soluble, water: 5 mg/mL

solubility

H2O: 20 mg/mL, PBS: insoluble

solubility

-

assay

≥98% (HPLC)

assay

≥90% (HPLC)

assay

≥98% (HPLC)

assay

>90%

Klasa składowania

11 - Combustible Solids

Co się dzieje?

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

PPE (środki ochrony indywidualnej)

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  

  1. Which filter sets can be used to visualize the fluorophore produced by Product D9564, DAPI, dilactate (DAPI-DNA)?

    1 answer

    1. One such filter set is the Zeiss Filter Set 01 (ex=359 nm, em=461 nm).

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. Can Product D9564, DAPI dilactate (DAPI-DNA), be used as a vital stain?

    1 answer

    1. As we note in the Product Information Sheet for the Dihydrochloride salt of DAPI, Product No. D9542, DAPI can be used as a vital dye to stain mature pollen grains, but we have tested it as a vital stain in other cell types.

      Helpful?

  4. Isn't the hydrochloride salt form of DAPI a more sensitive detector of DNA than Product D9564, DAPI, dilactate (DAPI-DNA)?

    1 answer

    1. The dilactate salt has as much sensitivity in detection as the dihydrochloride salt, which we also offer as Product No. D9542.

      Helpful?

1 Review This action will navigate to reviews.
1 of 1 reviewers received a sample product or took part in a promotion
1 out of 1 (100%) reviewers recommend this product

Reviews

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  1. St. Louis, MO
    • Review 1
    • Votes 0
    4 out of 5 stars.

    Good Quality

    Arrived quickly. Labeling is dimmer than expected.

    Helpful?