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Agarose

High EEO

Synonim(y):

3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE

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Rozmiar/SKUDostępnośćCena netto
50 g
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934,00 zł
700,50 zł
500 g
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
5930,00 zł
4447,50 zł

Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
41105317
PubChem Substance ID:
EC Number:
232-731-8
NACRES:
NA.25
MDL number:

700,50 zł

Cena katalogowa934,00 złZaoszczędź 25%

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grade

Molecular Biology

Quality Segment

form

powder

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

ign. residue

≤1%

loss

≤10% loss on drying

EEO

0.23-0.27

transition temp

gel point 34-37 °C (1.5% solution)

gel strength

≥1500 g/cm2

anion traces

chloride (Cl-): ≤0.3%, sulfate (SO42-): ≤0.6%

cation traces

Ca: ≤0.05%, Cd: ≤0.001%, Co: ≤0.001%, Cr: ≤0.001%, Cu: ≤0.001%, Fe: ≤0.001%, K: ≤0.05%, Mg: ≤0.001%, Mn: ≤0.001%, Na: ≤0.5%, Ni: ≤0.001%, Pb: ≤0.001%, Zn: ≤0.001%

SMILES string

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

Application

Agarose is used for preparative and analytical separation of nucleic acids. It can be used for single cell gel electrophoresis assay. It can also be used for southern blotting. It can also be used for assessing transepithelial ionic fluxes from cultured neonatal rat semicircular canal epithelium.

Biochem/physiol Actions

Agarose is a polysaccharide used for resolving DNA and RNA fragments from 500 - 20,000 bp. It provides strong gel structure which assists in better handling and less breakage. It can also reexpress the differentiated collagen phenotype from dedifferentiated chondrocytes during agarose gel culture.

Packaging

Packaging
50, 500 g in poly bottle

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
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A0169A2790A9793
description

High EEO

description

Type I-A, low EEO

description

Wide range, Molecular Biology

description

High EEO

form

powder

form

powder

form

powder

form

powder

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

grade

Molecular Biology

grade

-

grade

Molecular Biology

grade

-

anion traces

chloride (Cl-): ≤0.3%, sulfate (SO42-): ≤0.6%

anion traces

sulfate (SO42-): ≤0.20%

anion traces

sulfate (SO42-): ≤0.15%

anion traces

sulfate (SO42-): ≤0.25%

gel strength

≥1500 g/cm2

gel strength

≥1200 g/cm2 (1% gel)

gel strength

-

gel strength

≥750 g/cm2 (1% gel)

EEO

0.23-0.27

EEO

0.09-0.13

EEO

≤0.15

EEO

0.23-0.26


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Klasa składowania

11 - Combustible Solids

Co się dzieje?

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

PPE (środki ochrony indywidualnej)

Eyeshields, Gloves, type N95 (US)



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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