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05470

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, crystallized, ≥98.0% (GE)

Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
CAS Number:
Numer EC:
Numer MDL:
NACRES:
NA.27

pochodzenie biologiczne

bovine

Poziom jakości

200

próba

≥98.0% (GE)

forma

lyophilized powder

masa cząsteczkowa

~66 kDa

opakowanie

glass bottle of

technique(s)

cell culture | mammalian: suitable
electron microscopy: suitable

pH

4.8-7.5

rozpuszczalność

H2O: slightly soluble

ślady anionów

chloride (Cl-): ≤2000 ppm
sulfate (SO42-): ≤1000 ppm

ślady kationów

Ca: ≤500 ppm
Cd: ≤5 ppm
Co: ≤5 ppm
Cr: ≤100 ppm
Cu: ≤20 ppm
Fe: ≤20 ppm
K: ≤50 ppm
Mg: ≤500 ppm
Mn: ≤5 ppm
Na: ≤7000 ppm
Ni: ≤5 ppm
Pb: ≤5 ppm
Zn: ≤10 ppm

numer dostępu UniProt

temp. przechowywania

2-8°C

Gene Information

bovine ... ALB(280717)

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Zastosowanie

Albumin from bovine serum can be used for Electron and Immunoelectron microscopy. It can also be used for isolating and culturing neonatal rat cardiac ventricular myocytes.
Bovine serum albumin has been used to quantify proteins in raw enzyme extracts. It has also been used as a blocking agent for mitochondrial imaging.

Działania biochem./fizjol.

Bovine serum albumin also refers as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It can also trigger insulin dependent diabetes mellitus.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Opakowanie

Bottomless glass bottle. Contents are inside inserted fused cone.

Uwaga dotycząca przygotowania

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Inne uwagi

Review
Sales restrictions may apply

Kod klasy składowania

13 - Non Combustible Solids

WGK

WGK 3

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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