This product can be reconstituted by dissolving the lyophilized powder in an appropriate phosphate buffer or Tris-HCl buffer at a pH that matches the assay conditions. For testing purposes, this product is dissolved at 2 mg/mL in water. Mix gently until fully dissolved.
Przejdź do
19359
Glucose dehydrogenase from Pseudomonas sp.
powder, white, ≥200 U/mg
Cechy związane ze zrównoważonym rozwojem
Synonim(y):
GDH
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
10 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1210,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-836-9
MDL number:
Numer WE:
Specific activity:
≥200 U/mg
Biological source:
bacterial (Pseudomonas spp.)
1210,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
bacterial (Pseudomonas spp.)
Quality Segment
form
powder
specific activity
≥200 U/mg
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Sustainability features
color
white
storage temp.
−20°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biofuel cell research. For more information see the article in biofiles.
Application
Glucose dehydrogenase from Pseudomonas sp. has been used for the oxidation of glucose as a part of flow-injection analysis detection. It has also been used to regenerate the consumed nicotinamide adenine dinucleotide (NADH) during the removal of pyruvate by lactate dehydrogenase.
Biochem/physiol Actions
In bacteria, the membrane integrated glucose dehydrogenase catalyses the conversion of glucose to gluconic acid. It uses pyrroloquinoline quinone as a coenzyme. The intracellular glucose dehydrogenase lack this cofactor. It is a nicotinamide adenine dinucleotide (NADH) dependent enzyme.
Other Notes
One unit corresponds to the amount of enzyme which will oxidizes 1 μmole β-D-glucose to D-glucono-δ-lactone per minute at pH 8.0 and 37 °C
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Ta pozycja | |||
|---|---|---|---|
| description powder, white, ≥200 U/mg | description recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein (biuret) | description ammonium sulfate suspension, ≥10,000,000 units/mg protein | description lyophilized powder, ≥200 units/mg protein |
| specific activity ≥200 U/mg | specific activity ≥50 units/mg protein (biuret) | specific activity ≥10,000,000 units/mg protein | specific activity ≥200 units/mg protein |
| biological source bacterial (Pseudomonas spp.) | biological source Pseudomonas fluorescens | biological source - | biological source bacterial (Pseudomonas lemoignei) |
| form powder | form ammonium sulfate suspension | form ammonium sulfate suspension | form lyophilized powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| color white | color white | color - | color - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
Klasa składowania
11 - Combustible Solids
Co się dzieje?
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
Eyeshields, Gloves, type N95 (US)
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how to dissolve this lyophilized stock
1 answer-
Helpful?
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Could this GDH contain PQQ co-factor, or just NADH?
1 answer-
This product is not known to contain PQQ co-factor.
Helpful?
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Does this GDH contain FAD?
1 answer-
No, this product does not contain FAD and is not FAD-dependent. This product is Glucose 1-Dehydrogenase, as such this is an NAD dehydrogenase.
Helpful?
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Does this product contain co-factor NADH?
1 answer-
NADH is already present as a cofactor in this product, so there is no need to add it.
Helpful?
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What solvent can Product 19359, Glucose dehydrogenase be dissolved in? How should it be stored?
1 answer-
Glucose Dehydrogenase can be dissolved in a solvent such as a phosphate buffer or Tris-HCl buffer. The product must be stored in a cool, dry and well-ventilated place with the container tightly closed. Recommended Storage temperature is -20°C.
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