A protocol is not available for this compound.
Please see the link below to review a publication that may be helpful.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712457/
Przejdź do
35845
DCFHDA
BioReagent, suitable for fluorescence, ≥95% (HPLC)
Synonim(y):
2′,7′-Dichlorofluorescein diacetate, Diacetyldichlorofluorescein
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
5 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2450,00 zł |
Informacje o tej pozycji
Wzór empiryczny (zapis Hilla):
C24H14Cl2O7
Numer CAS:
Masa cząsteczkowa:
485.27
UNSPSC Code:
12352108
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
68488
2450,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócQuality Segment
description
suitable as a fluorogenic substrate for esterases
product line
BioReagent
assay
≥95% (HPLC)
form
powder or crystals
mol wt
485.27 g/mol
color
faint red, white to faint brown
mp
232-234 °C (lit.)
solubility
DMSO: soluble
fluorescence
λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)
suitability
suitable for fluorescence
storage temp.
2-8°C
SMILES string
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
InChI
1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3
InChI key
VQVUBYASAICPFU-UHFFFAOYSA-N
General description
2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.
Application
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Ta pozycja | |||
|---|---|---|---|
| description BioReagent, suitable for fluorescence, ≥95% (HPLC) | description Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress. | description BioReagent, suitable for fluorescence, ≥95% (TLC) | description suitable for use as an indicator in chloride titration, ~90% (TLC), powder |
| suitability suitable for fluorescence | suitability - | suitability suitable for fluorescence | suitability - |
| color white to faint brown, faint red | color - | color - | color - |
| fluorescence λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase) | fluorescence - | fluorescence λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0 | fluorescence - |
| form powder or crystals | form crystalline solid | form solid | form powder |
| solubility DMSO: soluble | solubility DMSO: 5 mg/mL | solubility H2O: soluble (pH ≥ 5), DMF: soluble, DMSO: soluble | solubility ethanol: 25 mg/mL |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Can I get the protocol? Thank you
1 answer-
Helpful?
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What is the shelf life of this product, as a powder, and once dissolved?
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
Stock solutions of this item in organic solvents are stable for at least 6 months if stored at -20°C.
Helpful?
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What are the optical properties of the hydrolysis product of 2´,7´-Dichlorofluorescein diacetate, Product 35845?
1 answer-
Excitation maximum is at 504 nm; emission maximum is at 529 nm (in 0.1 M Tris pH 8.0 following treatment with an esterase).
Helpful?
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In what solvents is 2´,7´-´Dichlorofluorescein diacetate, Product 35845, soluble?
1 answer-
It is soluble in DMF, ethanol (2 mg/mL), DMSO (10 mg/mL).
Helpful?
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What are the recommended storage conditions for 2´\,7´-Dichlorofluorescein diacetate, Product 35845?
1 answer-
Product no. 35845 should be stored in a refrigerator or coldroom at 2-8°C.
Helpful?
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Can Product 35845, 2´,7´-Dichlorofluorescein diacetate be used for ROS (Reactive Oxygen Species) detection?
1 answer-
Yes, Product 35845, 2´,7´-Dichlorofluorescein diacetate can be used for ROS detection by FACS analysis.
Helpful?
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What is required to get 2´,7´-Dichlorofluorescein diacetate, Product 35845, to fluoresce?
1 answer-
2',7'-dichlorofluorescein diacetate fluoresces only after cleavage of the acetyl groups.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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